ArticleDevelopment (Cambridge, England)2012
Zebrafish rest regulates developmental gene expression but not neurogenesis.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Pharmacological inhibition of RE1 silencing transcription factor disrupts SOX2 expression and neurogenesis in human induced pluripotent stem cells derived neuronal models.Metabolic brain disease · 2025Article
- REST Is Not Resting: REST/NRSF in Health and Disease.Biomolecules · 2023Review
- Development and migration of the zebrafish rhombencephalic octavolateral efferent neurons.The Journal of comparative neurology · 2021Article
- CoRest1 regulates neurogenesis in a stage-dependent manner.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- miR-124-3p is a chronic regulator of gene expression after brain injury.Cellular and molecular life sciences : CMLS · 2018Article
- Zebrafish sin3b mutants are viable but have size, skeletal, and locomotor defects.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Article
- The Importance of REST for Development and Function of Beta Cells.Frontiers in cell and developmental biology · 2017Review
- Brain REST/NRSF Is Not Only a Silent Repressor but Also an Active Protector.Molecular neurobiology · 2017Review
- Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Mariner Transposons Contain a Silencer: Possible Role of the Polycomb Repressive Complex 2.PLoS genetics · 2016Article
- Disruption of Rest Leads to the Early Onset of Cataracts with the Aberrant Terminal Differentiation of Lens Fiber Cells.PloS one · 2016Article
- REST represses a subset of the pancreatic endocrine differentiation program.Developmental biology · 2015Article
- Rest represses maturation within migrating facial branchiomotor neurons.Developmental biology · 2015Article
- Rest mutant zebrafish swim erratically and display atypical spatial preferences.Behavioural brain research · 2015Article
- Article
- The role of targeted protein degradation in early neural development.Genesis (New York, N.Y. : 2000) · 2014Review
- Facial motor neuron migration advances.Current opinion in neurobiology · 2013Review
- Integrative analysis of SF-1 transcription factor dosage impact on genome-wide binding and gene expression regulation.Nucleic acids research · 2013Article
- The nitroreductase system of inducible targeted ablation facilitates cell-specific regenerative studies in zebrafish.Methods (San Diego, Calif.) · 2013Article
- Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The transcriptional repressor Rest (Nrsf) recruits chromatin-modifying complexes to RE1 'silencer elements', which are associated with hundreds of neural genes. However, the requirement for Rest-mediated transcriptional regulation of embryonic development and cell fate is poorly understood. Conflicting views of the role of Rest in controlling cell fate have emerged from recent studies. To address these controversies, we examined the developmental requirement for Rest in zebrafish using zinc-finger nuclease-mediated gene targeting. We discovered that germ layer specification progresses normally in rest mutants despite derepression of target genes during embryogenesis. This analysis provides the first evidence that maternal rest is essential for repression of target genes during blastula stages. Surprisingly, neurogenesis proceeds largely normally in rest mutants, although abnormalities are observed within the nervous system, including defects in oligodendrocyte precursor cell development and a partial loss of facial branchiomotor neuron migration. Mutants progress normally through embryogenesis but many die as larvae (after 12 days). However, some homozygotes reach adulthood and are viable. We utilized an RE1/NRSE transgenic reporter system to dynamically monitor Rest activity. This analysis revealed that Rest is required to repress gene expression in mesodermal derivatives including muscle and notochord, as well as within the nervous system. Finally, we demonstrated that Rest is required for long-term repression of target genes in non-neural tissues in adult zebrafish. Our results point to a broad role for Rest in fine-tuning neural gene expression, rather than as a widespread regulator of neurogenesis or cell fate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.