ArticleJournal of proteome research2012
Online nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Leveraging HILIC/ERLIC separations for online nanoscale LC-MS/MS analysis of phosphopeptide isoforms from RNA polymerase II C-terminal domain.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2025Article
- Optimization of glycopeptide enrichment techniques for the identification of clinical biomarkers.Expert review of proteomics · 2024Review
- Advances in neuroproteomics for neurotrauma: unraveling insights for personalized medicine and future prospects.Frontiers in neurology · 2023Review
- Review
- O-glycosylation sites identified from mucin core-1 type glycopeptides from human serum.Glycoconjugate journal · 2016Article
- Sensing Small Changes in Protein Abundance: Stimulation of Caco-2 Cells by Human Whey Proteins.Journal of proteome research · 2016Article
- Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples.International journal of molecular sciences · 2015Review
- Quantitative Analysis of the Human Milk Whey Proteome Reveals Developing Milk and Mammary-Gland Functions across the First Year of Lactation.Proteomes · 2013Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- Genome-scale proteome quantification by DEEP SEQ mass spectrometry.Nature communications · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We have explored the use of electrostatic repulsion hydrophilic interaction chromatography (ERLIC) as an alternative to the gold-standard in shotgun proteomics: reversed-phase (RP) LC for online ESI-MS/MS. Conditions for sample solubilization and initial gradient conditions were optimized to strike a balance between peptide solubility and maximum peptide retention when using mobile phase with high organic solvent concentration. Online ERLIC-MS demonstrated a 57% increase in total peptide identifications compared to RP-MS. We examined the mechanism of this improved performance and found that it stems from ERLIC's propensity to retain longer peptides, which can be identified with greater confidence. Online nanoscale ERLIC-MS provides a powerful new tool for enhancing MS-based shotgun proteomic in a broad range of applications.
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Registered trials
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