Trial reportReproductive biology and endocrinology : RB&E2012
Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation.
Trial report in Reproductive biology and endocrinology : RB&E, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Serum Reproductive Hormone Profiles and Endometrial Expression of miR-16-5p,Advanced biomedical research · 2025Article
- The role of epigenetic mechanisms in the regulation of gene expression in the cyclical endometrium.Clinical epigenetics · 2021Review
- Effect of parental and ART treatment characteristics on perinatal outcomes.Human reproduction (Oxford, England) · 2021Article
- Secreted MicroRNA to Predict Embryo Implantation Outcome: From Research to Clinical Diagnostic Application.Frontiers in cell and developmental biology · 2020Review
- MicroRNA Biogenesis Machinery Is Dysregulated in the Endometrium of Infertile Women Suggesting a Role in Receptivity and Infertility.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Epigenetics: A key paradigm in reproductive health.Clinical and experimental reproductive medicine · 2016Review
- Human Blastocyst Secreted microRNA Regulate Endometrial Epithelial Cell Adhesion.EBioMedicine · 2015Article
- Joint MiRNA/mRNA expression profiling reveals changes consistent with development of dysfunctional corpus luteum after weight gain.PloS one · 2015Article
- MicroRNAs induced in melanoma treated with combination targeted therapy of Temsirolimus and Bevacizumab.Journal of translational medicine · 2013Article
- Endometrial Expression of Angiogenesis-related Factors Following Ovarian Stimulation and Epigallocatechin Gallate Administration.Iranian journal of nursing and midwifery researchArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStudies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles.
methodsThe study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3-5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel.
resultsA total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p < 0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3-5 days after retrieval. During the peri-implantation period (3-5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group.
conclusionLuteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes.
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