ArticleMicrobial cell factories2012
Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Adaptability ofCombinatorial chemistry & high throughput screening · 2026Article
- Engineering Embden-Meyerhof-Parnas Glycolysis to Generate Noncanonical Reducing Power.ACS catalysis · 2022Article
- Article
- Plant Synthetic Metabolic Engineering for Enhancing Crop Nutritional Quality.Plant communications · 2020Review
- Developing a single strain for in vitro salvage synthesis of NADMicrobial cell factories · 2019Article
- Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid.Frontiers in bioengineering and biotechnology · 2019Article
- AnSynthetic and systems biotechnology · 2018Review
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- AnApplied and environmental microbiology · 2017Article
- Modules forSynthetic and systems biotechnology · 2017Review
- A synthetic biochemistry platform for cell free production of monoterpenes from glucose.Nature communications · 2017Article
- Biomanufacturing: history and perspective.Journal of industrial microbiology & biotechnology · 2017Review
- Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis.ACS synthetic biology · 2016Article
- Forward design of a complex enzyme cascade reaction.Nature communications · 2016Article
- Insight into thermophiles and their wide-spectrum applications.3 Biotech · 2016Review
- Stability of Ensemble Models Predicts Productivity of Enzymatic Systems.PLoS computational biology · 2016Article
- Cell-free metabolic engineering: biomanufacturing beyond the cell.Biotechnology journal · 2015Review
- Building carbon-carbon bonds using a biocatalytic methanol condensation cycle.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A synthetic biochemistry system for the in vitro production of isoprene from glycolysis intermediates.Protein science : a publication of the Protein Society · 2014Article
- Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol.AMB Express · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe integration of biotechnology into chemical manufacturing has been recognized as a key technology to build a sustainable society. However, the practical applications of biocatalytic chemical conversions are often restricted due to their complexities involving the unpredictability of product yield and the troublesome controls in fermentation processes. One of the possible strategies to overcome these limitations is to eliminate the use of living microorganisms and to use only enzymes involved in the metabolic pathway. Use of recombinant mesophiles producing thermophilic enzymes at high temperature results in denaturation of indigenous proteins and elimination of undesired side reactions; consequently, highly selective and stable biocatalytic modules can be readily prepared. By rationally combining those modules together, artificial synthetic pathways specialized for chemical manufacturing could be designed and constructed.
resultsA chimeric Embden-Meyerhof (EM) pathway with balanced consumption and regeneration of ATP and ADP was constructed by using nine recombinant E. coli strains overproducing either one of the seven glycolytic enzymes of Thermus thermophilus, the cofactor-independent phosphoglycerate mutase of Pyrococcus horikoshii, or the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase of Thermococcus kodakarensis. By coupling this pathway with the Thermus malate/lactate dehydrogenase, a stoichiometric amount of lactate was produced from glucose with an overall ATP turnover number of 31.
conclusionsIn this study, a novel and simple technology for flexible design of a bespoke metabolic pathway was developed. The concept has been testified via a non-ATP-forming chimeric EM pathway. We designated this technology as "synthetic metabolic engineering". Our technology is, in principle, applicable to all thermophilic enzymes as long as they can be functionally expressed in the host, and thus would be potentially applicable to the biocatalytic manufacture of any chemicals or materials on demand.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.