ArticleInternational journal of molecular sciences2012
RNA-mediated gene silencing signals are not graft transmissible from the rootstock to the scion in greenhouse-grown apple plants Malus sp.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Molecular mechanisms driving the success of grafting impacted by walnut rootstock.BMC genomics · 2026Article
- Bridging the Gap: Genetic Insights into Graft Compatibility for Enhanced Kiwifruit Production.International journal of molecular sciences · 2025Review
- Article
- Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions.Frontiers in plant science · 2023Article
- Effect of Grafting on Viral Resistance of Non-transgenic Plum Scion Combined With Transgenic PPV-Resistant Rootstock.Frontiers in plant science · 2021Article
- An artificial miRNA as a new tool to silence and explore gene functions in apple.Transgenic research · 2019Article
- RNAi-Mediated Resistance Against Viruses in Perennial Fruit Plants.Plants (Basel, Switzerland) · 2019Review
- Article
- In vitro gene expression and mRNA translocation from transformed walnut (Juglans regia) rootstocks expressing DsRED fluorescent protein to wild-type scions.Plant cell reports · 2017Article
- The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica).Plant biotechnology journal · 2016Article
- Fighting Sharka in Peach: Current Limitations and Future Perspectives.Frontiers in plant science · 2016Article
- Biotechnology and apple breeding in Japan.Breeding science · 2016Review
- Unravelling rootstock×scion interactions to improve food security.Journal of experimental botany · 2015Review
- Biosafety considerations of RNAi-mediated virus resistance in fruit-tree cultivars and in rootstock.Transgenic research · 2013Review
- Graft transmission of RNA silencing to non-transgenic scions for conferring virus resistance in tobacco.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing describes the sequence specific degradation of RNA targets. Silencing is a non-cell autonomous event that is graft transmissible in different plant species. The present study is the first report on systemic acquired dsRNA-mediated gene silencing of transgenic and endogenous gene sequences in a woody plant like apple. Transgenic apple plants overexpressing a hairpin gene construct of the gusA reporter gene were produced. These plants were used as rootstocks and grafted with scions of the gusA overexpressing transgenic apple clone T355. After grafting, we observed a reduction of the gusA gene expression in T355 scions in vitro, but not in T355 scions grown in the greenhouse. Similar results were obtained after silencing of the endogenous Mdans gene in apple that is responsible for anthocyanin biosynthesis. Subsequently, we performed grafting experiments with Mdans silenced rootstocks and red leaf scions of TNR31-35 in order to evaluate graft transmitted silencing of the endogenous Mdans. The results obtained suggested a graft transmission of silencing signals in in vitro shoots. In contrast, no graft transmission of dsRNA-mediated gene silencing signals was detectable in greenhouse-grown plants and in plants grown in an insect protection tent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.