ArticleCold Spring Harbor protocols2012
Circuit mapping by ultraviolet uncaging of glutamate.
Article in Cold Spring Harbor protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 27 citations in OpenAlex.
- Pyramidal cell types and circuit organization of the mouse insular cortex reveal functional specializations.Nature neuroscience · 2026Article
- Neuronal architecture of the mouse insular cortex underlying its diverse functions.bioRxiv : the preprint server for biology · 2025Article
- Review
- What Can We Learn from Synaptic Connectivity Maps about Cerebellar Internal Models?Cerebellum (London, England) · 2023Review
- Propagating wave activity in a tangential cortical slice.Neuroreport · 2020Article
- Optical control of neuronal ion channels and receptors.Nature reviews. Neuroscience · 2019Review
- Thalamocortical and Intracortical Inputs Differentiate Layer-Specific Mouse Auditory Corticocollicular Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- High-yield in vitro recordings from neurons functionally characterized in vivo.Nature protocols · 2018Article
- Two-Photon Uncaging of Glutamate.Frontiers in synaptic neuroscience · 2018Review
- Two-color, one-photon uncaging of glutamate and GABA.PloS one · 2017Article
- Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits.Cold Spring Harbor protocols · 2016Article
- Mapping Auditory Synaptic Circuits with Photostimulation of Caged Glutamate.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Commissural functional topography of the inferior colliculus assessed in vitro.Hearing research · 2015Article
- Nicotinic alteration of functional thalamocortical topography.Neuroreport · 2015Article
- Synaptic circuit organization of motor corticothalamic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Article
- Frequency transformation in the auditory lemniscal thalamocortical system.Frontiers in neural circuits · 2014Review
- Laser-scanning photostimulation of optogenetically targeted forebrain circuits.Journal of visualized experiments : JoVE · 2013Article
- eGFP expression under UCHL1 promoter genetically labels corticospinal motor neurons and a subpopulation of degeneration-resistant spinal motor neurons in an ALS mouse model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Laminarly orthogonal excitation of fast-spiking and low-threshold-spiking interneurons in mouse motor cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
In laser photostimulation, small clusters of neurons in brain slices are induced to fire action potentials by focal glutamate uncaging, and synaptic connectivity between photoexcited presynaptic neurons and individual postsynaptic neurons is assessed by intracellular recording of synaptic events. With a scanner, this process can be repeated sequentially across a patterned array of stimulus locations, generating maps of neurons' local sources of synaptic inputs. Laser scanning photostimulation (LSPS) based on patterned glutamate uncaging offers an efficient, quantitative, optical-electrophysiological way to map synaptic circuits in brain slices. The efficacy of glutamate-based photostimulation for circuit mapping (in contrast to electrical stimulation) derives from the ability to stimulate neurons with high precision and speed, and without stimulating axons of passage. This protocol describes the components, assembly, and operation of a laser scanning microscope for ultraviolet (UV) uncaging, along with experimental methods for circuit mapping in brain slices. It presents a general approach and a set of guidelines for quantitative circuit mapping using "standard" LSPS methods based on single-photon glutamate uncaging using a UV laser, a pair of scanning mirror galvanometers, a patch-clamp setup, and open-source data acquisition software.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.