ArticleProceedings of the National Academy of Sciences of the United States of America2012
Tension induces a base-paired overstretched DNA conformation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Determinants of dicentric chromosome breakage inbioRxiv : the preprint server for biology · 2026Article
- Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics.Nucleic acids research · 2025Article
- Stacking correlation length in single-stranded DNA.Nucleic acids research · 2024Article
- Understanding protein diffusion on force-induced stretched DNA conformation.Frontiers in molecular biosciences · 2022Article
- Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy.The journal of physical chemistry. B · 2021Article
- Mismatch detection in homologous strand exchange amplified by hydrophobic effects.Biopolymers · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- GC-Content Dependence of Elastic and Overstretching Properties of DNA:RNA Hybrid Duplexes.Biophysical journal · 2020Article
- Stretching DNA to twice the normal length with single-molecule hydrodynamic trapping.Lab on a chip · 2020Article
- A Tour de Force on the Double Helix: Exploiting DNA Mechanics To Study DNA-Based Molecular Machines.Biochemistry · 2019Review
- Weaving DNA strands: structural insight on ATP hydrolysis in RecA-induced homologous recombination.Nucleic acids research · 2019Article
- Hydrophobic catalysis and a potential biological role of DNA unstacking induced by environment effects.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nanomechanics of Diaminopurine-Substituted DNA.Biophysical journal · 2019Article
- Single-molecule polarization microscopy of DNA intercalators sheds light on the structure of S-DNA.Science advances · 2019Article
- SNARE machinery is optimized for ultrafast fusion.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers.Biophysical journal · 2019Article
- Light-Responsive Polymer Particles as Force Clamps for the Mechanical Unfolding of Target Molecules.Nano letters · 2018Article
- Optical Trapping Nanometry of Hypermethylated CPG-Island DNA.Biophysical journal · 2017Article
- Single-molecule studies of high-mobility group B architectural DNA bending proteins.Biophysical reviews · 2017Review
- An AT-barrier mechanically controls DNA reannealing under tension.Nucleic acids research · 2016Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mixed-sequence DNA molecules undergo mechanical overstretching by approximately 70% at 60-70 pN. Since its initial discovery 15 y ago, a debate has arisen as to whether the molecule adopts a new form [Cluzel P, et al. (1996) Science 271:792-794; Smith SB, Cui Y, Bustamante C (1996) Science 271:795-799], or simply denatures under tension [van Mameren J, et al. (2009) Proc Natl Acad Sci USA 106:18231-18236]. Here, we resolve this controversy by using optical tweezers to extend small 60-64 bp single DNA duplex molecules whose base content can be designed at will. We show that when AT content is high (70%), a force-induced denaturation of the DNA helix ensues at 62 pN that is accompanied by an extension of the molecule of approximately 70%. By contrast, GC-rich sequences (60% GC) are found to undergo a reversible overstretching transition into a distinct form that is characterized by a 51% extension and that remains base-paired. For the first time, results proving the existence of a stretched basepaired form of DNA can be presented. The extension observed in the reversible transition coincides with that produced on DNA by binding of bacterial RecA and human Rad51, pointing to its possible relevance in homologous recombination.
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