ArticleProceedings of the National Academy of Sciences of the United States of America2012
Direct substitution and assisted dissociation pathways for turning off transcription by a MerR-family metalloregulator.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A dynamic duo: Copper metalloregulators and continuous-wave electron spin resonance spectroscopy.Biophysical reports · 2026Review
- Electron Paramagnetic Resonance Spectroscopy Reveals Promoter Dependent Transcription Regulation by Copper Activated CueR in Pseudomonas aeruginosa.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Conformation-gated binding underlies kinetic asymmetry and negative cooperativity in ATP:cob(I)alamin adenosyltransferase.Cell reports. Physical science · 2025Article
- A 'through-DNA' mechanism for co-regulation of metal uptake and efflux.Nature communications · 2024Article
- The Copper Efflux Regulator (CueR).Sub-cellular biochemistry · 2024Review
- Fine-tuning gene expression of regulator AdmX for improved biosynthesis of andrimid in Erwinia persicina BST187.Applied microbiology and biotechnology · 2023Article
- Meddling with Metal Sensors: Fur-Family Proteins as Signaling Hubs.Journal of bacteriology · 2023Review
- EPR Spectroscopy Provides New Insights into Complex Biological Reaction Mechanisms.The journal of physical chemistry. B · 2022Review
- The use of EPR spectroscopy to study transcription mechanisms.Biophysical reviews · 2022Review
- Unique underlying principles shaping copper homeostasis networks.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Review
- Allostery-driven changes in dynamics regulate the activation of bacterial copper transcription factor.Protein science : a publication of the Protein Society · 2022Article
- Facilitated dissociation of nucleoid-associated proteins from DNA in the bacterial confinement.Biophysical journal · 2022Article
- Bacterial Transcriptional Regulators: A Road Map for Functional, Structural, and Biophysical Characterization.International journal of molecular sciences · 2022Review
- Cu(ii)-based DNA labeling identifies the structural link between transcriptional activation and termination in a metalloregulator.Chemical science · 2022Article
- The molecular basis of allostery in a facilitated dissociation process.Structure (London, England : 1993) · 2021Article
- Single-molecule conformational dynamics of a transcription factor reveals a continuum of binding modes controlling association and dissociation.Nucleic acids research · 2021Article
- On the specificity of protein-protein interactions in the context of disorder.The Biochemical journal · 2021Review
- Article
- Polyelectrolyte interactions enable rapid association and dissociation in high-affinity disordered protein complexes.Nature communications · 2020Article
- Dissection of Interaction Kinetics through Single-Molecule Interaction Simulation.Analytical chemistry · 2020Article
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Authors and funding
8 authors.
Funding
Abstract
Metalloregulators regulate transcription in response to metal ions. Many studies have provided insights into how transcription is activated upon metal binding by MerR-family metalloregulators. In contrast, how transcription is turned off after activation is unclear. Turning off transcription promptly is important, however, as the cells would not want to continue expressing metal resistance genes and thus waste energy after metal stress is relieved. Using single-molecule FRET measurements we studied the dynamic interactions of the copper efflux regulator (CueR), a Cu(+)-responsive MerR-family metalloregulator, with DNA. Besides quantifying its DNA binding and unbinding kinetics, we discovered that CueR spontaneously flips its binding orientation at the recognition site. CueR also has two different binding modes, corresponding to interactions with specific and nonspecific DNA sequences, which would facilitate recognition localization. Most strikingly, a CueR molecule coming from solution can directly substitute for a DNA-bound CueR or assist the dissociation of the incumbent CueR, both of which are unique examples for any DNA-binding protein. The kinetics of the direct protein substitution and assisted dissociation reactions indicate that these two unique processes can provide efficient pathways to replace a DNA-bound holo-CueR with apo-CueR, thus turning off transcription promptly and facilely.
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