ArticleStem cells (Dayton, Ohio)2012
Cellular heterogeneity during embryonic stem cell differentiation to epiblast stem cells is revealed by the ShcD/RaLP adaptor protein.
Article in Stem cells (Dayton, Ohio), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Variant U1 snRNAs contribute to cell cycle and differentiation control of human iPS cells.Nature communications · 2026Article
- Immune escape and metastasis mechanisms in melanoma: breaking down the dichotomy.Frontiers in immunology · 2024Review
- Three-Dimensional Microtumor Formation of Infantile Hemangioma-Derived Endothelial Cells for Mechanistic Exploration and Drug Screening.Pharmaceuticals (Basel, Switzerland) · 2022Article
- SHC4 promotes tumor proliferation and metastasis by activating STAT3 signaling in hepatocellular carcinoma.Cancer cell international · 2022Article
- ShcD Binds DOCK4, Promotes Ameboid Motility and Metastasis Dissemination, Predicting Poor Prognosis in Melanoma.Cancers · 2020Article
- Localization of phosphotyrosine adaptor protein ShcD/SHC4 in the adult rat central nervous system.BMC neuroscience · 2019Article
- Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.Oncology reports · 2018Article
- Network Features and Dynamical Landscape of Naive and Primed Pluripotency.Biophysical journal · 2018Article
- Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation.Scientific reports · 2017Article
- The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states.BMC developmental biology · 2017Review
- Insights into the Shc Family of Adaptor Proteins.Journal of molecular signaling · 2017Article
- Eat, breathe, ROS: controlling stem cell fate through metabolism.Expert review of cardiovascular therapy · 2017Review
- Biologically active substances-enriched diet regulates gonadotrope cell activation pathway in liver of adult and old rats.Genes & nutrition · 2014Article
- The ShcD signaling adaptor facilitates ligand-independent phosphorylation of the EGF receptor.Molecular biology of the cell · 2014Article
- Heterogeneity of the level of activity of lgr5+ intestinal stem cells.International journal of molecular and cellular medicine · 2014Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Shc family of adaptor proteins are crucial mediators of a plethora of receptors such as the tyrosine kinase receptors, cytokine receptors, and integrins that drive signaling pathways governing proliferation, differentiation, and migration. Here, we report the role of the newly identified family member, ShcD/RaLP, whose expression in vitro and in vivo suggests a function in embryonic stem cell (ESC) to epiblast stem cells (EpiSCs) transition. The transition from the naïve (ESC) to the primed (EpiSC) pluripotent state is the initial important step for ESCs to commit to differentiation and the mechanisms underlying this process are still largely unknown. Using a novel approach to simultaneously assess pluripotency, apoptosis, and proliferation by multiparameter flow cytometry, we show that ESC to EpiSC transition is a process involving a tight coordination between the modulation of the Oct4 expression, cell cycle progression, and cell death. We also describe, by high-content immunofluorescence analysis and time-lapse microscopy, the emergence of cells expressing caudal-related homeobox 2 (Cdx2) transcription factor during ESC to EpiSC transition. The use of the ShcD knockout ESCs allowed the unmasking of this process as they presented deregulated Oct4 modulation and an enrichment in Oct4-negative Cdx2-positive cells with increased MAPK/extracellular-regulated kinases 1/2 activation, within the differentiating population. Collectively, our data reveal ShcD as an important modulator in the switch of key pathway(s) involved in determining EpiSC identity.
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