ArticleActa crystallographica. Section D, Biological crystallography2012
Spatial distribution of radiation damage to crystalline proteins at 25-300 K.
Article in Acta crystallographica. Section D, Biological crystallography, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Separating the effects of temperature and absorbed X-ray dose on unit-cell volume.Acta crystallographica. Section D, Structural biology · 2026Article
- Pinpointing Protein Crystal Structures over a Broad Temperature Range Using Hydrophobic Protection.ACS omega · 2026Article
- Temperature-Resolved Crystallography Reveals Rigid-Body Dominance over Local Flexibility in B‑Factors.ACS omega · 2025Article
- Evaluating the impact of X-ray damage on conformational heterogeneity in room-temperature (277 K) and cryo-cooled protein crystals.Acta crystallographica. Section D, Structural biology · 2022Article
- Solvent flows, conformation changes and lattice reordering in a cold protein crystal.Acta crystallographica. Section D, Structural biology · 2019Article
- Solving a new R2lox protein structure by microcrystal electron diffraction.Science advances · 2019Article
- Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography.Acta crystallographica. Section D, Structural biology · 2019Article
- Conformational variation of proteins at room temperature is not dominated by radiation damage.Journal of synchrotron radiation · 2017Article
- Mapping the conformational landscape of a dynamic enzyme by multitemperature and XFEL crystallography.eLife · 2015Article
- Identifying and quantifying radiation damage at the atomic level.Journal of synchrotron radiation · 2015Review
- Temperature-dependent radiation sensitivity and order of 70S ribosome crystals.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Identification of patterns in diffraction intensities affected by radiation exposure.Journal of synchrotron radiation · 2013Article
- Global radiation damage: temperature dependence, time dependence and how to outrun it.Journal of synchrotron radiation · 2013Article
- Sumner's legacy: a century of urease crystals and recent structural advances.Critical reviews in biochemistry and molecular biologyReview
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4 authors.
Funding
Abstract
The spatial distribution of radiation damage (assayed by increases in atomic B factors) to thaumatin and urease crystals at temperatures ranging from 25 to 300 K is reported. The nature of the damage changes dramatically at approximately 180 K. Above this temperature the role of solvent diffusion is apparent in thaumatin crystals, as solvent-exposed turns and loops are especially sensitive. In urease, a flap covering the active site is the most sensitive part of the molecule and nearby loops show enhanced sensitivity. Below 180 K sensitivity is correlated with poor local packing, especially in thaumatin. At all temperatures, the component of the damage that is spatially uniform within the unit cell accounts for more than half of the total increase in the atomic B factors and correlates with changes in mosaicity. This component may arise from lattice-level, rather than local, disorder. The effects of primary structure on radiation sensitivity are small compared with those of tertiary structure, local packing, solvent accessibility and crystal contacts.
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