Evidence map›Paper›PMID 22947857›Full record

ArticleBiophysical journal2012

Distinct kinetic and molecular requirements govern CD44 binding to hyaluronan versus fibrin(ogen).

Phrabha S Raman, Christina S Alves, Denis Wirtz, Konstantinos Konstantopoulos

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 28 citations in OpenAlex.

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  7. Review
  8. Article
  9. A mode of cell adhesion and migration facilitated by CD44-dependent microtentacles.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  10. Article
  11. Article
  12. E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017
    Article
  13. Article
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  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Phrabha S RamanDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland.
Christina S AlvesDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland.
Denis WirtzDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, Maryland; Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, Maryland; Center of Cancer Nanotechnology Excellence, The Johns Hopkins University, Baltimore, Maryland.
Konstantinos KonstantopoulosDepartment of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, Maryland; Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, Maryland; Center of Cancer Nanotechnology Excellence, The Johns Hopkins University, Baltimore, Maryland. Electronic address: kkonsta1@jhu.edu.
Johns Hopkins University · US

Funding

TRANS NETWORK PROJECTSU54CA143868 · NCI · JOHNS HOPKINS UNIVERSITY · PI SEMENZA, GREGG L · 2009 to 2013
$13.7M
Molecular Characterization of Cell-Cell Interactions in Cancer MetastasisR01CA101135 · NCI · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS · 2003 to 2011
$2.5M
NCI NIH HHS R01 CA101135NCI NIH HHS U54 CA143868
6 · The paper itself

Abstract

CD44 is a multifunctional glycoprotein that binds to hyaluronan and fibrin(ogen). Alternative splicing is responsible for the generation of numerous different isoforms, the smallest of which is CD44s. Insertion of variant exons into the extracellular membrane proximal region generates the variant isoforms (CD44v). Here, we used force spectroscopy to delineate the biophysical and molecular requirements of CD44-HA and CD44-fibrin(ogen) interactions at the single-molecule level. CD44v-HA and CD44s-HA single bonds exhibit similar kinetic and micromechanical properties because the HA-binding motif on CD44 is common to all of the isoforms. Although this is the primary binding site, O- and N-linked glycans and sulfation also contribute to the tensile strength of the CD44-HA bond. The CD44s-fibrin pair has a lower unstressed dissociation rate and a higher tensile strength than CD44s-fibrinogen but is weaker than the CD44-HA bond. In contrast to CD44-HA binding, the molecular interaction between CD44 and fibrin(ogen) is predominantly mediated by the chondroitin sulfate and dermatan sulfate on CD44. Blocking sulfation on CD44s modestly decreases the tensile strength of CD44s-fibrin(ogen) binding, which is in stark contrast to CD44v-fibrin interaction. Collectively, the results obtained by force spectroscopy in conjunction with biochemical interventions enable us to delineate the biophysical parameters and molecular constituents of CD44 binding to hyaluronan and fibrin(ogen).

Indexed as

Biomechanical PhenomenaCell Line, TumorFibrinFibrinogenGlycosylationHumansHyaluronan ReceptorsHyaluronic AcidKineticsProtein BindingProtein IsoformsFibrinFibrinogenHyaluronan ReceptorsHyaluronic AcidProtein Isoforms

Identifiers

PMID22947857
PMCPMC3414901
OpenAlexW1981639170

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.