ArticleBiophysical journal2012
Atomic force microscopy stiffness tomography on living Arabidopsis thaliana cells reveals the mechanical properties of surface and deep cell-wall layers during growth.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 131 citations in OpenAlex.
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- Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.BMC plant biology · 2019Article
- Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A inPlants (Basel, Switzerland) · 2019Article
- Tensile Testing Assay for the Measurement of Tissue Stiffness inBio-protocol · 2019Article
- Assessment of Primary Cell Wall Nanomechanical Properties in Internal Cells of Non-Fixed Maize Roots.Plants (Basel, Switzerland) · 2019Article
- Discrete mechanical growth model for plant tissue.PloS one · 2019Article
- Cell Wall Polymer Composition and Spatial Distribution in Ripe Banana and Mango Fruit: Implications for Cell Adhesion and Texture Perception.Frontiers in plant science · 2019Article
- High-Throughput Analysis of Arabidopsis Stem Vibrations to Identify Mutants With Altered Mechanical Properties.Frontiers in plant science · 2018Article
- Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy.Scientific reports · 2017Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used atomic force microscopy (AFM) stiffness tomography to explore stiffness distribution in the cell wall of suspension-cultured Arabidopsis thaliana as a model of primary, growing cell wall. For the first time that we know of, this new imaging technique was performed on living single cells of a higher plant, permitting monitoring of the stiffness distribution in cell-wall layers as a function of the depth and its evolution during the different growth phases. The mechanical measurements were correlated with changes in the composition of the cell wall, which were revealed by Fourier-transform infrared (FTIR) spectroscopy. In the beginning and end of cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, whereas in the exponential growth phase, the average wall stiffness increased, with increasing heterogeneity. In this phase, the difference between the superficial and deep wall stiffness was highest. FTIR spectra revealed a relative increase in the polysaccharide/lignin content.
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