ArticleJournal of biological engineering2012
Evolutionary principles and synthetic biology: avoiding a molecular tragedy of the commons with an engineered phage.
Article in Journal of biological engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Gut Microbiota and Metabolic Syndrome: Relationships and Opportunities for New Therapeutic Strategies.Scientifica · 2024Review
- Microbiome therapeutics: exploring the present scenario and challenges.Gastroenterology report · 2022Review
- Engineered Bacteriophage Therapeutics: Rationale, Challenges and Future.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Methods for measuring the evolutionary stability of engineered genomes to improve their longevity.Synthetic biology (Oxford, England) · 2021Article
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- Article
- On the stability of sequences inserted into viral genomes.Virus evolution · 2019Review
- Complementary and Alternative Medicine Strategies for Therapeutic Gut Microbiota Modulation in Inflammatory Bowel Disease and their Next-Generation Approaches.Gastroenterology clinics of North America · 2017Review
- Restoring logic and data to phage-cures for infectious disease.AIMS microbiology · 2017Review
- Microbiome therapeutics - Advances and challenges.Advanced drug delivery reviews · 2016Review
- Design and engineering of a transmissible antiviral defense.Journal of biological engineering · 2016Article
- Evolutionary decay and the prospects for long-term disease intervention using engineered insect vectors.Evolution, medicine, and public health · 2015Review
- Bacteriophages and phage-derived proteins--application approaches.Current medicinal chemistry · 2015Review
- Differential codon adaptation between dsDNA and ssDNA phages in Escherichia coli.Molecular biology and evolution · 2014Article
- Bacteriophage-based synthetic biology for the study of infectious diseases.Current opinion in microbiology · 2014Article
- The habits of highly effective phages: population dynamics as a framework for identifying therapeutic phages.Frontiers in microbiology · 2014Article
- Article
- Challenges in predicting the evolutionary maintenance of a phage transgene.Journal of biological engineering · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
unlabelled
backgroundIn prior work, adding a gene to phage T7 that degraded the host K1 capsule facilitated growth when plated on capsulated hosts. However, the transgenic protein (an endosialidase) is expressed as an exoenzyme, released from the cell at lysis but unattached to the phage particle. There is thus the possibility that the gene will be subject to a tragedy of the commons and be selected against, if the enzyme benefits other genomes.
resultsThis evolutionary perspective was supported in short term experiments. The genome carrying the endosialidase gene was favored on a capsulated host if grown in physical isolation of control genomes (lacking the gene) but was selected against otherwise.
conclusionsThese results challenge efforts to engineer phages with exoenzymes that degrade biofilm polymers. If biofilms do not facilitate spatially structured phage growth, the transgenic enzymes may be rapidly eliminated from the phage population after release in the environment, even if the transgene benefits overall phage growth on the biofilm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.