Evidence map›Paper›PMID 22947061›Full record

ReviewCellular microbiology2012

Superresolution microscopy for microbiology.

Carla Coltharp, Jie Xiao

Open access · bronzeAbstract readReview
In one paragraph

Review in Cellular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
10.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 123 citations in OpenAlex.

  1. Case Study: Illuminating the Nanoscale World of Microbiology.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021
    Review
  13. Article
  14. Supramolecular assembly of theProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Carla ColtharpDepartment of Biophysics & Biophysical Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jie Xiao
Johns Hopkins University · US

Funding

Probing the structure, dynamics and function of the E. coli divisomeR01GM086447 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI XIAO, JIE · 2011 to 2019
$3.8M
NIGMS NIH HHS R01 GM086447
6 · The paper itself

Abstract

This review provides a practical introduction to superresolution microscopy from the perspective of microbiological research. Because of the small sizes of bacterial cells, superresolution methods are particularly powerful and suitable for revealing details of cellular structures that are not resolvable under conventional fluorescence light microscopy. Here we describe the methodological concepts behind three major categories of superresolution light microscopy: photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) and stimulated emission-depletion (STED) microscopy. We then present recent applications of each of these techniques to microbial systems, which have revealed novel conformations of cellular structures and described new properties of in vivo protein function and interactions. Finally, we discuss the unique issues related to implementing each of these superresolution techniques with bacterial specimens and suggest avenues for future development. The goal of this review is to provide the necessary technical background for interested microbiologists to choose the appropriate superresolution method for their biological systems, and to introduce the practical considerations required for designing and analysing superresolution imaging experiments.

Indexed as

Biomedical ResearchMicrobiological TechniquesMicroscopy

Identifiers

PMID22947061
PMCPMC3894611
OpenAlexW2146394719

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.