ArticleJournal of experimental botany2012
PPR8522 encodes a chloroplast-targeted pentatricopeptide repeat protein necessary for maize embryogenesis and vegetative development.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 65 citations in OpenAlex.
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Analysis of candidate genes identified via genome-wide association analysis of sugar-related traits in maize kernels.The plant genome · 2025Article
- Article
- Discovery of candidate genes enhancing kernel protein content in tropical maize introgression lines.BMC plant biology · 2024Article
- Molecular mechanisms controlling grain size and weight and their biotechnological breeding applications in maize and other cereal crops.Journal of advanced research · 2024Review
- Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.Horticulture research · 2024Article
- Integrated analysis of BSA-seq and RNA-seq identified the candidate genes for seed weight inFrontiers in plant science · 2024Article
- Functional Differences of Grapevine Circular RNAPlants (Basel, Switzerland) · 2023Article
- A Systemic Investigation of Genetic Architecture and Gene Resources Controlling Kernel Size-Related Traits in Maize.International journal of molecular sciences · 2023Article
- The Italian Research on the Molecular Characterization of Maize Kernel Development.International journal of molecular sciences · 2022Review
- Functions of PPR Proteins in Plant Growth and Development.International journal of molecular sciences · 2021Review
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Emerging Roles of RNA-Binding Proteins in Seed Development and Performance.International journal of molecular sciences · 2020Review
- Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum.The Plant cell · 2020Article
- Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize.Methods in molecular biology (Clifton, N.J.) · 2020Article
- An essential pentatricopeptide repeat protein in the apicomplexan remnant chloroplast.Cellular microbiology · 2019Article
- Pentatricopeptide repeat protein DEK40 is required for mitochondrial function and kernel development in maize.Journal of experimental botany · 2019Article
- Genome-Wide Analysis of the DYW Subgroup PPR Gene Family and Identification ofInternational journal of molecular sciences · 2019Article
- Novel Cytonuclear Combinations ModifyFrontiers in plant science · 2019Article
- Genome-wide analysis of the pentatricopeptide repeat gene family in different maize genomes and its important role in kernel development.BMC plant biology · 2018Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pentatricopeptide repeat (PPR) domain is an RNA binding domain allowing members of the PPR superfamily to participate in post-transcriptional processing of organellar RNA. Loss of PPR8522 from maize (Zea mays) confers an embryo-specific (emb) phenotype. The emb8522 mutation was isolated in an active Mutator (Mu) population and co-segregation analysis revealed that it was tightly linked to a MuDR insertion in the first exon of PPR8522. Independent evidence that disruption of PPR8522 caused the emb phenotype was provided by fine mapping to a region of 116kb containing no other gene than PPR8522 and complementation of the emb8522 mutant by a PPR8522 cDNA. The deduced PPR8522 amino acid sequence of 832 amino acids contains 10 PPR repeats and a chloroplast target peptide, the function of which was experimentally demonstrated by transient expression in Nicotiana benthamiana. Whereas mutant endosperm is apparently normal, mutant embryos deviate from normal development as early as 3 days after pollination, are reduced in size, exhibit more or less severe morphological aberrations depending on the genetic background, and generally do not germinate. The emb8522 mutation is the first to associate the loss of a PPR gene with an embryo-lethal phenotype in maize. Analyses of mutant plantlets generated by embryo-rescue experiments indicate that emb8522 also affects vegetative plant growth and chloroplast development. The loss of chloroplast transcription dependent on plastid-encoded RNA polymerase is the likely cause for the lack of an organized thylakoid network and an albino, seedling-lethal phenotype.
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