ArticleScandinavian journal of gastroenterology2012
Isolation and characterization of human primary enterocytes from small intestine using a novel method.
Article in Scandinavian journal of gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine.iScience · 2026Article
- Intestinal Epithelial MHC-II at the Interface of Microbiota, Immunity, and Inflammation.International journal of molecular sciences · 2026Review
- Detailed survey of aniScience · 2024Article
- A novel oral formulation of the melanocortin-1 receptor agonist PL8177 resolves inflammation in preclinical studies of inflammatory bowel disease and is gut restricted in rats, dogs, and humans.Frontiers in immunology · 2023Article
- Article
- Calcium-sensing receptor is not expressed in the absorptive enterocytes of weaned piglets.Journal of animal science · 2022Article
- Mitochondrial and glycolytic extracellular flux analysis optimization for isolated pig intestinal epithelial cells.Scientific reports · 2021Article
- Sodium Butyrate ReducesFrontiers in microbiology · 2020Article
- TLR10 Senses HIV-1 Proteins and Significantly Enhances HIV-1 Infection.Frontiers in immunology · 2019Article
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Biology open · 2018Article
- Predicting the murine enterocyte metabolic response to diets that differ in lipid and carbohydrate composition.Scientific reports · 2017Article
- Contribution of in vitro comparison of colorectal carcinoma cells from primary and metastatic lesions to elucidation of mechanisms of tumor progression and response to anticancer therapy.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes.Oxidative medicine and cellular longevity · 2016Article
- Glucose transport by epithelia prepared from harvested enterocytes.Cytotechnology · 2015Article
- Predicting the impact of diet and enzymopathies on human small intestinal epithelial cells.Human molecular genetics · 2013Article
- Recellularization of acellular human small intestine using bone marrow stem cells.Stem cells translational medicine · 2013Article
- Differential contributions of the gut microbiota and metabolome to pathomechanisms in ulcerative colitis: anGut microbesArticle
Corrections and comments
- Correction · 2016-06-01Error in Image ·
- Erratum issuedCorrigendum.2016
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell culture studies of enterocytes are important in many fields. However, there are difficulties in obtaining cell lines from adult human intestine, such as microbial contamination of cultures from the tissue samples, short life span of enterocytes, overgrowth of mesenchymal cells, etc. Various model used to obtain adult intestinal cell lines are very complex requiring use of feeder layer or gel matrices. The aim of this study was to establish a novel method for the simple and reproducible isolation of human enterocytes. Enterocytes were isolated from SI samples (n = 5) obtained from cadaveric donors using a mechanical procedure, and separation with immunomagnetic beads coated with anti-EpCAM antibodies. Light and electron microscopy, flow cytometry and immunocytochemistry techniques were used to characterize the isolated cells. Immunohistochemical staining of normal SB biopsies confirmed that the cell cultures maintained an in vivo phenotype as reflected in cytokeratin expression CK18, CK20 and expression of intestine-specific markers such as sucrase isomaltase and maltase glucoamylase. Furthermore, the cells strongly expressed TLR-5, 6, 7, 8 and 10 and several molecules such as CD40, CD86, CD44, ICAM-1 and HLA-DR which are important in triggering cell-mediated immune responses. This novel technique provides a unique in vitro system to study the biology of enterocytes in normal conditions as well as to study inflammatory processes in various small bowel disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.