ArticleThe Plant cell2012
Ribulose-1,5-bis-phosphate carboxylase/oxygenase accumulation factor1 is required for holoenzyme assembly in maize.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 121 citations in OpenAlex.
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- Hornworts reveal a spatial model for pyrenoid-based CONature plants · 2025Article
- COI1 F-box proteins regulate DELLA protein levels, growth, and photosynthetic efficiency in maize.The Plant cell · 2024Article
- Comparative Proteomic Analysis of Floral Buds before and after Opening in Walnut (International journal of molecular sciences · 2024Article
- Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize-Peanut Intercropping System.Plants (Basel, Switzerland) · 2023Article
- Red Rubiscos and opportunities for engineering green plants.Journal of experimental botany · 2023Review
- Structural insights into cyanobacterial RuBisCO assembly coordinated by two chaperones Raf1 and RbcX.Cell discovery · 2022Article
- Overexpression ofGenes · 2022Article
- Improving the efficiency of Rubisco by resurrecting its ancestors in the family Solanaceae.Science advances · 2022Article
- Rubiscosome gene expression is balanced across the hexaploid wheat genome.Photosynthesis research · 2022Article
- The state of oligomerization of Rubisco controls the rate of synthesis of the Rubisco large subunit in Chlamydomonas reinhardtii.The Plant cell · 2021Article
- Chloroplast Chaperonin-Mediated Targeting of a Thylakoid Membrane Protein.The Plant cell · 2020Article
- Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli.Nature plants · 2020Article
- A Synthetic Cytokinin Improves Photosynthesis in Rice under Drought Stress by Modulating the Abundance of Proteins Related to Stomatal Conductance, Chlorophyll Contents, and Rubisco Activity.Plants (Basel, Switzerland) · 2020Article
- Rubisco accumulation factor 1 (Raf1) plays essential roles in mediating Rubisco assembly and carboxysome biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- OsCpn60β1 is Essential for Chloroplast Development in Rice (International journal of molecular sciences · 2020Article
- Increased Rubisco content in maize mitigates chilling stress and speeds recovery.Plant biotechnology journal · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most life is ultimately sustained by photosynthesis and its rate-limiting carbon fixing enzyme, ribulose-1,5-bis-phosphate carboxylase/oxygenase (Rubisco). Although the structurally comparable cyanobacterial Rubisco is amenable to in vitro assembly, the higher plant enzyme has been refractory to such manipulation due to poor understanding of its assembly pathway. Here, we report the identification of a chloroplast protein required for Rubisco accumulation in maize (Zea mays), RUBISCO ACCUMULATION FACTOR1 (RAF1), which lacks any characterized functional domains. Maize lines lacking RAF1 due to Mutator transposon insertions are Rubisco deficient and seedling lethal. Analysis of transcripts and proteins showed that Rubisco large subunit synthesis in raf1 plants is not compromised; however, newly synthesized Rubisco large subunit appears in a high molecular weight form whose accumulation requires a specific chaperonin 60 isoform. Gel filtration analysis and blue native gels showed that endogenous and recombinant RAF1 are trimeric; however, following in vivo cross-linking, RAF1 copurifies with Rubisco large subunit, suggesting that they interact weakly or transiently. RAF1 is predominantly expressed in bundle sheath chloroplasts, consistent with a Rubisco accumulation function. Our results support the hypothesis that RAF1 acts during Rubisco assembly by releasing and/or sequestering the large subunit from chaperonins early in the assembly process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.