ArticleThe Journal of biological chemistry2012
Expression profiling and biochemical analysis suggest stress response as a potential mechanism inhibiting proliferation of polyamine-depleted cells.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Solid-state cultivation-specific agmatine production by Aspergillus oryzae: current understanding and perspectives.Amino acids · 2026Review
- Epigenetic crosstalk in neuroblastoma development and progression.Frontiers in cell and developmental biology · 2026Review
- Dietary supplements and vascular function in hypertensive disorders of pregnancy.Pflugers Archiv : European journal of physiology · 2023Review
- Fructose Consumption Alters Biogenic Amines Associated with Cardiovascular Disease Risk Factors.Arquivos brasileiros de cardiologia · 2023Article
- SLC3A2 and SLC7A2 Mediate the Exogenous Putrescine-Induced Adipocyte Differentiation.Molecules and cells · 2022Article
- Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.Nucleic acids research · 2022Article
- New Insights into the Roles and Mechanisms of Spermidine in Aging and Age-Related Diseases.Aging and disease · 2021Review
- Polyamines: Functions, Metabolism, and Role in Human Disease Management.Medical sciences (Basel, Switzerland) · 2021Review
- ATP13A2-mediated endo-lysosomal polyamine export counters mitochondrial oxidative stress.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Polyamines Mediate Folding of Primordial Hyperacidic Helical Proteins.Biochemistry · 2020Article
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- Mapping Metabolic Events in the Cancer Cell Cycle Reveals Arginine Catabolism in the Committed SGCell reports · 2019Article
- Dietary and Gut Microbiota Polyamines in Obesity- and Age-Related Diseases.Frontiers in nutrition · 2019Review
- Roles of polyamines in translation.The Journal of biological chemistry · 2018Review
- Inducing controlled cell cycle arrest and re-entry during asexual proliferation of Plasmodium falciparum malaria parasites.Scientific reports · 2018Article
- Polyamine Metabolism and Oxidative Protein Folding in the ER as ROS-Producing Systems Neglected in Virology.International journal of molecular sciences · 2018Review
- Tracing the dynamics of gene transcripts after organismal death.Open biology · 2017Article
- Targeting ornithine decarboxylase (ODC) inhibits esophageal squamous cell carcinoma progression.NPJ precision oncology · 2017Article
- Cellular Homeostasis and Antioxidant Response in Epithelial HT29 Cells on Titania Nanotube Arrays Surface.Oxidative medicine and cellular longevity · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Polyamines are small organic polycations that are absolutely required for cell growth and proliferation; yet the basis for this requirement is mostly unknown. Here, we combined a genome-wide expression profiling with biochemical analysis to reveal the molecular basis for inhibited proliferation of polyamine-depleted cells. Transcriptional responses accompanying growth arrest establishment in polyamine-depleted cells or growth resumption following polyamine replenishment were monitored and compared. Changes in the expression of genes related to various fundamental cellular processes were established. Analysis of mirror-symmetric expression patterns around the G(1)-arrest point identified a set of genes representing a stress-response signature. Indeed, complementary biochemical analysis demonstrated activation of the PKR-like endoplasmic reticulum kinase arm of the unfolded protein response and of the stress-induced p38 MAPK. These changes were accompanied by induction of key growth-inhibitory factors such as p21 and Gadd45a and reduced expression of various cyclins, most profoundly cyclin D1, setting the basis for the halted proliferation. However, although the induced stress response could arrest growth, polyamine depletion also inhibited proliferation of PKR-like endoplasmic reticulum kinase and p38α-deficient cells and of cells harboring a nonphosphorylatable mutant eIF2α (S51A), suggesting that additional yet unidentified mechanisms might inhibit proliferation of polyamine-depleted cells. Despite lengthy persistence of the stress and activation of apoptotic signaling, polyamine-depleted cells remained viable, apparently due to induced expression of protective genes and development of autophagy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.