ArticleNucleic acids research2012
Complementary strand relocation may play vital roles in RecA-based homology recognition.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Flanking strand separation activity of RecA nucleoprotein filaments in DNA strand exchange reactions.Nucleic acids research · 2023Article
- Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination.Nucleic acids research · 2021Article
- Micro-homology intermediates: RecA's transient sampling revealed at the single molecule level.Nucleic acids research · 2021Article
- Mismatch sensing by nucleofilament deciphers mechanism of RecA-mediated homologous recombination.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51.Nature communications · 2020Article
- Integrating multi-scale data on homologous recombination into a new recognition mechanism based on simulations of the RecA-ssDNA/dsDNA structure.Nucleic acids research · 2015Article
- An Overview of the Molecular Mechanisms of Recombinational DNA Repair.Cold Spring Harbor perspectives in biology · 2015Review
- The poor homology stringency in the heteroduplex allows strand exchange to incorporate desirable mismatches without sacrificing recognition in vivo.Nucleic acids research · 2015Article
- DNA strand exchange and RecA homologs in meiosis.Cold Spring Harbor perspectives in biology · 2014Review
- RecA-mediated sequence homology recognition as an example of how searching speed in self-assembly systems can be optimized by balancing entropic and enthalpic barriers.Physical review. E, Statistical, nonlinear, and soft matter physics · 2014Article
- Mechanisms and principles of homology search during recombination.Nature reviews. Molecular cell biology · 2014Review
- RecA-dependent programmable endonuclease Ref cleaves DNA in two distinct steps.Nucleic acids research · 2014Article
- The differential extension in dsDNA bound to Rad51 filaments may play important roles in homology recognition and strand exchange.Nucleic acids research · 2014Article
- Simplified biased random walk model for RecA-protein-mediated homology recognition offers rapid and accurate self-assembly of long linear arrays of binding sites.Physical review. E, Statistical, nonlinear, and soft matter physics · 2013Article
- Tension on dsDNA bound to ssDNA-RecA filaments may play an important role in driving efficient and accurate homology recognition and strand exchange.Physical review. E, Statistical, nonlinear, and soft matter physics · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
RecA-family proteins mediate homologous recombination and recombinational DNA repair through homology search and strand exchange. Initially, the protein forms a filament with the incoming single-stranded DNA (ssDNA) bound in site I. The RecA-ssDNA filament then binds double-stranded DNA (dsDNA) in site II. Non-homologous dsDNA rapidly unbinds, whereas homologous dsDNA undergoes strand exchange yielding heteroduplex dsDNA in site I and the leftover outgoing strand in site II. We show that applying force to the ends of the complementary strand significantly retards strand exchange, whereas applying the same force to the outgoing strand does not. We also show that crystallographically determined binding site locations require an intermediate structure in addition to the initial and final structures. Furthermore, we demonstrate that the characteristic dsDNA extension rates due to strand exchange and free RecA binding are the same, suggesting that relocation of the complementary strand from its position in the intermediate structure to its position in the final structure limits both rates. Finally, we propose that homology recognition is governed by transitions to and from the intermediate structure, where the transitions depend on differential extension in the dsDNA. This differential extension drives strand exchange forward for homologs and increases the free energy penalty for strand exchange of non-homologs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.