ArticleNucleic acids research2012
Yeast CAF-1 assembles histone (H3-H4)2 tetramers prior to DNA deposition.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 78 citations in OpenAlex.
- HAT1 Regulates Intestinal Stem Cell Proliferation and Differentiation.bioRxiv : the preprint server for biology · 2026Article
- H3K56 acetylation regulates chromatin maturation following DNA replication.Nature communications · 2025Article
- Unorthodox PCNA Binding by Chromatin Assembly Factor 1.International journal of molecular sciences · 2022Article
- Genome-wide Identification, Expression, and Functional Analysis ofFrontiers in genetics · 2022Article
- Epigenetic regulation of nuclear lamina-associated heterochromatin by HAT1 and the acetylation of newly synthesized histones.Nucleic acids research · 2021Article
- Review
- CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.PLoS genetics · 2020Article
- Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 inGenetics · 2019Article
- ASF1a inhibition induces p53-dependent growth arrest and senescence of cancer cells.Cell death & disease · 2019Article
- Article
- Inheritance of CENP-A Nucleosomes during DNA Replication Requires HJURP.Developmental cell · 2018Article
- Mechanistic insights into histone deposition and nucleosome assembly by the chromatin assembly factor-1.Nucleic acids research · 2018Review
- SUMO2 conjugation of PCNA facilitates chromatin remodeling to resolve transcription-replication conflicts.Nature communications · 2018Article
- Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast.Nucleic acids research · 2018Article
- ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.Molecular cell · 2017Article
- Identification of multiple roles for histone acetyltransferase 1 in replication-coupled chromatin assembly.Nucleic acids research · 2017Article
- Breaking Symmetry - Asymmetric Histone Inheritance in Stem Cells.Trends in cell biology · 2017Review
- A Molecular Prospective for HIRA Complex Assembly and H3.3-Specific Histone Chaperone Function.Journal of molecular biology · 2017Review
- Article
- Histone chaperone networks shaping chromatin function.Nature reviews. Molecular cell biology · 2017Review
Corrections and comments
- Retraction · 2017-08-18Error in Analyses · Error in Data · Error in Results and/or Conclusions ·
- Retracted
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Following acetylation, newly synthesized H3-H4 is directly transferred from the histone chaperone anti-silencing factor 1 (Asf1) to chromatin assembly factor 1 (CAF-1), another histone chaperone that is critical for the deposition of H3-H4 onto replicating DNA. However, it is unknown how CAF-1 binds and delivers H3-H4 to the DNA. Here, we show that CAF-1 binds recombinant H3-H4 with 10- to 20-fold higher affinity than H2A-H2B in vitro, and H3K56Ac increases the binding affinity of CAF-1 toward H3-H4 2-fold. These results provide a quantitative thermodynamic explanation for the specific H3-H4 histone chaperone activity of CAF-1. Surprisingly, H3-H4 exists as a dimer rather than as a canonical tetramer at mid-to-low nanomolar concentrations. A single CAF-1 molecule binds a cross-linked (H3-H4)2 tetramer, or two H3-H4 dimers that contain mutations at the (H3-H4)2 tetramerization interface. These results suggest that CAF-1 binds to two H3-H4 dimers in a manner that promotes formation of a (H3-H4)2 tetramer. Consistent with this idea, we confirm that CAF-1 synchronously binds two H3-H4 dimers derived from two different histone genes in vivo. Together, the data illustrate a clear mechanism for CAF-1-associated H3-H4 chaperone activity in the context of de novo nucleosome (re)assembly following DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.