ArticleApplied and environmental microbiology2012
Enhancement of ganoderic acid accumulation by overexpression of an N-terminally truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in the basidiomycete Ganoderma lucidum.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 99 citations in OpenAlex.
- Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.Molecular biotechnology · 2026Article
- Comprehensive genomic identification and functional analysis ofChinese herbal medicines · 2026Article
- Blue Light Receptor WC-2 Regulates Ganoderic Acid Biosynthesis inJournal of fungi (Basel, Switzerland) · 2025Article
- CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities.World journal of microbiology & biotechnology · 2025Review
- Gene Editing inJournal of fungi (Basel, Switzerland) · 2025Review
- Exogenous ethephon treatment on the biosynthesis and accumulation of astragaloside IV in Astragalus membranaceus Bge. Var. Mongholicus (Bge.) Hsiao.Botanical studies · 2024Article
- Molecular Characterization and Expression Analysis of a Gene Encoding 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGR) fromJournal of fungi (Basel, Switzerland) · 2024Article
- Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects.Journal of fungi (Basel, Switzerland) · 2024Review
- Review
- Breeding a newFrontiers in microbiology · 2024Article
- The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments.Scientific reports · 2023Article
- Comparative transcriptome analysis of genes and metabolic pathways involved in sporulation in Ganoderma lingzhi.G3 (Bethesda, Md.) · 2022Article
- Establishment of an Efficient Polyethylene Glycol (PEG)-Mediated Transformation System inJournal of fungi (Basel, Switzerland) · 2022Article
- A methyltransferase LaeA regulates ganoderic acid biosynthesis inFrontiers in microbiology · 2022Article
- Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways.Frontiers in plant science · 2022Article
- Biosynthesis and regulation of terpenoids from basidiomycetes: exploration of new research.AMB Express · 2021Review
- Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9.Applied and environmental microbiology · 2021Article
- The non-canonical functions of telomerase reverse transcriptase gene GlTert on regulating fungal growth, oxidative stress, and ganoderic acid biosynthesis in Ganoderma lucidum.Applied microbiology and biotechnology · 2021Article
- Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum.Microbial cell factories · 2021Article
- Effects of Efficient Expression ofMicroorganisms · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Ganoderic acids produced by Ganoderma lucidum, a well-known traditional Chinese medicinal mushroom, exhibit antitumor and antimetastasis activities. Genetic modification of G. lucidum is difficult but critical for the enhancement of cellular accumulation of ganoderic acids. In this study, a homologous genetic transformation system for G. lucidum was developed for the first time using mutated sdhB, encoding the iron-sulfur protein subunit of succinate dehydrogenase, as a selection marker. The truncated G. lucidum gene encoding the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was overexpressed by using the Agrobacterium tumefaciens-mediated transformation system. The results showed that the mutated sdhB successfully conferred carboxin resistance upon transformation. Most of the integrated transfer DNA (T-DNA) appeared as a single copy in the genome. Moreover, deregulated constitutive overexpression of the HMGR gene led to a 2-fold increase in ganoderic acid content. It also increased the accumulation of intermediates (squalene and lanosterol) and the upregulation of downstream genes such as those of farnesyl pyrophosphate synthase, squalene synthase, and lanosterol synthase. This study demonstrates that transgenic basidiomycete G. lucidum is a promising system to achieve metabolic engineering of the ganoderic acid pathway.
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