ArticleApplied and environmental microbiology2012
EDTA inhibits biofilm formation, extracellular vesicular secretion, and shedding of the capsular polysaccharide glucuronoxylomannan by Cryptococcus neoformans.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Calcium triggersbioRxiv : the preprint server for biology · 2026Article
- Membrane Vesicles ImproveMicroorganisms · 2026Article
- The biofilm produced by Cryptococcus neoformans protects the fungus from the antifungal and anti-melanin effects of cyclosporine.Microbial pathogenesis · 2025Article
- Improvement of the Lyophilization Survival Rate ofFoods (Basel, Switzerland) · 2022Article
- The phosphate language of fungi.Trends in microbiology · 2022Review
- Mycological Investigation of Bottled Water Dispensers in Healthcare Facilities.Pathogens (Basel, Switzerland) · 2021Article
- Extracellular DNA, cell surface proteins and c-di-GMP promote biofilm formation in Clostridioides difficile.Scientific reports · 2021Article
- Ethylenediaminetetraacetic Acid Disodium Salt Acts as an Antifungal Candidate Molecule againstToxins · 2020Article
- Pathogenic Delivery: The Biological Roles of Cryptococcal Extracellular Vesicles.Pathogens (Basel, Switzerland) · 2020Review
- N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii.BMC research notes · 2020Article
- Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives.Frontiers in cellular and infection microbiology · 2020Review
- Membrane Vesicle Production as a Bacterial Defense Against Stress.Frontiers in microbiology · 2020Review
- NLRP3 Inflammasome and Inflammatory Diseases.Oxidative medicine and cellular longevity · 2020Review
- Natamycin and Voriconazole Exhibit Synergistic Interactions with Nonantifungal Ophthalmic Agents againstAntimicrobial agents and chemotherapy · 2019Article
- Cryptococcal Traits Mediating Adherence to Biotic and Abiotic Surfaces.Journal of fungi (Basel, Switzerland) · 2018Review
- Investigation of Cryptococcus neoformans magnesium transporters reveals important role of vacuolar magnesium transporter in regulating fungal virulence factors.MicrobiologyOpen · 2018Article
- In vitro antibacterial effects of Tanreqing injection combined with vancomycin or linezolid against methicillin-resistant Staphylococcus aureus.BMC complementary and alternative medicine · 2018Article
- Enhanced Biofilm Formation and Membrane Vesicle Release byFrontiers in microbiology · 2018Article
- The Crucial Role of Biofilms in Cryptococcus neoformans Survival within Macrophages and Colonization of the Central Nervous System.Journal of fungi (Basel, Switzerland) · 2017Review
- In Vitro Approach for Identification of the Most Effective Agents for Antimicrobial Lock Therapy in the Treatment of Intravascular Catheter-Related Infections Caused by Staphylococcus aureus.Antimicrobial agents and chemotherapy · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The fungal pathogen Cryptococcus neoformans can grow as a biofilm on a range of synthetic and prosthetic materials. Cryptococcal biofilm formation can complicate the placement of shunts used to relieve increased intracranial pressure in cryptococcal meningitis and can serve as a nidus for chronic infection. Biofilms are generally advantageous to pathogens in vivo, as they can confer resistance to antimicrobial compounds, including fluconazole and voriconazole in the case of C. neoformans. EDTA can inhibit biofilm formation by several microbes and enhances the susceptibility of biofilms to antifungal drugs. In this study, we evaluated the effect of sublethal concentrations of EDTA on the growth of cryptococcal biofilms. EDTA inhibited biofilm growth by C. neoformans, and the inhibition could be reversed by the addition of magnesium or calcium, implying that the inhibitory effect was by divalent cation starvation. EDTA also reduced the amount of the capsular polysaccharide glucuronoxylomannan shed into the biofilm matrix and decreased vesicular secretion from the cell, thus providing a potential mechanism for the inhibitory effect of this cation-chelating compound. Our data imply that the growth of C. neoformans biofilms requires the presence of divalent metals in the growth medium and suggest that cations are required for the export of materials needed for biofilm formation, possibly including extracellular vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.