ArticleApplied and environmental microbiology2012
Autotrophic production of stable-isotope-labeled arginine in Ralstonia eutropha strain H16.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 54 citations in OpenAlex.
- Characterization and Crystal Structure Elucidation of the l-Homoserine O-Succinyltransferase MetXS From Cupriavidus necator H16.Chembiochem : a European journal of chemical biology · 2026Article
- The generation game: Toward the generational genetic stability of continuous culture.iScience · 2025Article
- HMicrobial cell factories · 2024Article
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
- Stable Platform for Mevalonate Bioproduction from COACS sustainable chemistry & engineering · 2024Article
- Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16.Microbial cell factories · 2024Article
- Expanding the synthetic biology toolbox of Cupriavidus necator for establishing fatty acid production.Journal of industrial microbiology & biotechnology · 2024Article
- COMicrobial cell factories · 2024Article
- Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium.Molecules (Basel, Switzerland) · 2022Article
- Lab-Scale Cultivation ofBioengineering (Basel, Switzerland) · 2022Article
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
- Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Hydrogen oxidising bacteria for production of single-cell protein and other food and feed ingredients.Engineering biology · 2020Review
- Low temperature-induced viable but not culturable state of Ralstonia eutropha and its relationship to accumulated polyhydroxybutyrate.FEMS microbiology letters · 2016Article
- Evaluation of gene expression cassettes and production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a fine modulated monomer composition by using it in Cupriavidus necator.Microbial cell factories · 2016Article
- RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha.The FEBS journal · 2016Article
- Metabolic engineering of microorganisms for the production of L-arginine and its derivatives.Microbial cell factories · 2014Article
- Structure, function and biosynthesis of O₂-tolerant hydrogenases.Nature reviews. Microbiology · 2013Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the aim of improving industrial-scale production of stable-isotope (SI)-labeled arginine, we have developed a system for the heterologous production of the arginine-containing polymer cyanophycin in recombinant strains of Ralstonia eutropha under lithoautotrophic growth conditions. We constructed an expression plasmid based on the cyanophycin synthetase gene (cphA) of Synechocystis sp. strain PCC6308 under the control of the strong P(cbbL) promoter of the R. eutropha H16 cbb(c) operon (coding for autotrophic CO(2) fixation). In batch cultures growing on H(2) and CO(2) as sole sources of energy and carbon, respectively, the cyanophycin content of cells reached 5.5% of cell dry weight (CDW). However, in the absence of selection (i.e., in antibiotic-free medium), plasmid loss led to a substantial reduction in yield. We therefore designed a novel addiction system suitable for use under lithoautotrophic conditions. Based on the hydrogenase transcription factor HoxA, this system mediated stabilized expression of cphA during lithoautotrophic cultivation without the need for antibiotics. The maximum yield of cyanophycin was 7.1% of CDW. To test the labeling efficiency of our expression system under actual production conditions, cells were grown in 10-liter-scale fermentations fed with (13)CO(2) and (15)NH(4)Cl, and the (13)C/(15)N-labeled cyanophycin was subsequently extracted by treatment with 0.1 M HCl; 2.5 to 5 g of [(13)C/(15)N]arginine was obtained per fed-batch fermentation, corresponding to isotope enrichments of 98.8% to 99.4%.
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