ArticleExperimental & molecular medicine2012
Apical acidity decreases inhibitory effect of omeprazole on Mg(2+) absorption and claudin-7 and -12 expression in Caco-2 monolayers.
Article in Experimental & molecular medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 42 citations in OpenAlex.
- A barrier-centered perspective on the gut-barrier-bone axis: a review of the core role of the intestinal barrier and related probiotics with nanocoatings.Archives of microbiology · 2026Review
- Proton pump inhibitor increases intestinal epithelial paracellular permeability via the p38-MAPK/NF-κB signaling pathway.Molecular biology reports · 2025Article
- Case Report: Multiple electrolyte disturbance with severe neurological manifestations induced by chronic use of PPIs.Frontiers in medicine · 2025Article
- Multiple electrolyte disorders triggered by proton pump inhibitor-induced hypomagnesemia: Case reports with a mini-review of the literature.Clinical nephrology. Case studies · 2024Article
- Acute Oncologic Complications: Clinical-Therapeutic Management in Critical Care and Emergency Departments.Current oncology (Toronto, Ont.) · 2023Review
- Hypomagnesemia and Cardiovascular Risk in Type 2 Diabetes.Endocrine reviews · 2023Article
- Current opinion on the regulation of small intestinal magnesium absorption.World journal of gastroenterology · 2023Review
- Proton pump inhibitors may enhance the risk of digestive diseases by regulating intestinal microbiota.Frontiers in pharmacology · 2023Review
- Mechanisms of proton pump inhibitor-induced hypomagnesemia.Acta physiologica (Oxford, England) · 2022Review
- Synthesis, Characterization, and Cellular Uptake of a Glycylglycine Chelate of Magnesium.ACS omega · 2021Article
- A New Caco-2 Cell Model of in Vitro Intestinal Barrier: Application for the Evaluation of Magnesium Salts Absorption.Physiological research · 2021Article
- Synthesis, Characterization, and Cellular Uptake of Magnesium Maltol and Ethylmaltol Complexes.ACS omega · 2021Article
- Prevention of hypomagnesemia in critically ill patients with acute kidney injury on continuous kidney replacement therapy: the role of early supplementation and close monitoring.Journal of nephrology · 2021Review
- Synthesis and Chemical and Biological Evaluation of a Glycine Tripeptide Chelate of Magnesium.Molecules (Basel, Switzerland) · 2021Article
- The Coordination Chemistry of Bio-Relevant Ligands and Their Magnesium Complexes.Molecules (Basel, Switzerland) · 2020Review
- Effect of dietaryAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2020Article
- The Use of Proton Pump Inhibitors May Increase Symptoms of Muscle Function Loss in Patients with Chronic Illnesses.International journal of molecular sciences · 2020Review
- The inhibitory role of purinergic P2Y receptor on MgThe journal of physiological sciences : JPS · 2019Article
- Challenges in the Diagnosis of Magnesium Status.Nutrients · 2018Review
- Proton Pump Inhibitor Usage and the Risk of Mortality in Hemodialysis Patients.Kidney international reports · 2018Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clinical studies reported hypomagnesaemia in long-term omeprazole usage that was probably due to intestinal Mg(2+) wasting. Our previous report demonstrated the inhibitory effect of omeprazole on passive Mg(2+) transport across Caco-2 monolayers. The present study aimed to identify the underlying mechanism of omeprazole suppression of passive Mg(2+) absorption. By using Caco-2 monolayers, we demonstrated a potent inhibitory effect of omeprazole on passive Mg(2+), but not Ca(2+), transport across Caco-2 monolayers. Omeprazole shifted the %maximum passive Mg(2+) transport-Mg(2+) concentration curves to the right, and increased the half maximal effective concentration of those dose-response curves, indicating a lower Mg(2+) affinity of the paracellular channel. By continually monitoring the apical pH, we showed that omeprazole suppressed apical acid accumulation. Neomycin and spermine had no effect on passive Mg(2+) transport of either control or omeprazole treated monolayers, indicating that omeprazole suppressed passive Mg(2+) transport in a calcium sensing receptor (CaSR)-independent manner. The results of western blot analysis showed that omeprazole significantly suppressed claudin (Cldn)-7 and -12, but not Cldn-2, expression in Caco-2 cells. By using apical solution of pH 5.5, 6.0, 6.5, and 7.0, we found that apical acidity markedly increased passive Mg(2+) transport, Mg(2+) affinity of the paracellular channel, and Cldn-7 and -12 expression in Caco-2 monolayers. Apical acidity abolished the inhibitory effect of omeprazole on passive Mg(2+) transport and Cldn-7 and -12 expression. Our results provided the evidence for the regulation of intestinal passive Mg(2+) absorption by luminal acidity-induced increase in Cldn-7 and -12 expression.
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