ArticleJournal of microbiological methods2012
Adaptation of the neutral bacterial comet assay to assess antimicrobial-mediated DNA double-strand breaks in Escherichia coli.
Article in Journal of microbiological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Influence of pH and lysis duration on DNA damage detection: a comparison of neutral and alkaline comet assays.Mutagenesis · 2025Article
- Targeting ESKAPE pathogens with ZnS and Au@ZnS Core-Shell nanoconjugates for improved biofilm control.Scientific reports · 2025Article
- Effect of Pefloxacin onAntibiotics (Basel, Switzerland) · 2025Article
- Membrane depolarization kills dormant Bacillus subtilis cells by generating a lethal dose of ROS.Nature communications · 2024Article
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- Exploring the Role of the NO-Detoxifying Enzyme HmpA in the Evolution of Domesticated Alfalfa Rhizobia.Microbial ecology · 2022Article
- Antibacterial Potential by Rupture Membrane and Antioxidant Capacity of Purified Phenolic Fractions ofAntibiotics (Basel, Switzerland) · 2021Article
- Article
- Nanoscale battery cathode materials induce DNA damage in bacteria.Chemical science · 2020Article
- IL-26 mediated human cell activation and antimicrobial activity againstCurrent research in microbial sciences · 2020Article
- Biological impact of nanoscale lithium intercalating complex metal oxides to model bacteriumEnvironmental science. Nano · 2019Article
- Evaluation of DNA damage in tobacco male meiocytes involved in cytomixis using comet assay.Protoplasma · 2018Article
- Bacterial lipid droplets bind to DNA via an intermediary protein that enhances survival under stress.Nature communications · 2017Article
- Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.Methods in enzymology · 2017Article
- DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This study aimed to determine the mechanism of action of a natural antibacterial clay mineral mixture, designated as CB, by investigating the induction of DNA double-strand breaks (DSBs) in Escherichia coli. To quantify DNA damage upon exposure to soluble antimicrobial compounds, we modified a bacterial neutral comet assay, which associates the general length of an electrophoresed chromosome, or comet, with the degree of DSB-associated DNA damage. To appropriately account for antimicrobial-mediated strand fragmentation, suitable control reactions consisting of exposures to water, ethanol, kanamycin, and bleomycin were developed and optimized for the assay. Bacterial exposure to the CB clay resulted in significantly longer comet lengths, compared to water and kanamycin exposures, suggesting that the induction of DNA DSBs contributes to the killing activity of this antibacterial clay mineral mixture. The comet assay protocol described herein provides a general technique for evaluating soluble antimicrobial-derived DNA damage and for comparing DNA fragmentation between experimental and control assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.