ArticleEpidemiology and infection2013
Evaluation of the pooling of swabs for real-time PCR detection of low titre shedding of low pathogenicity avian influenza in turkeys.
Article in Epidemiology and infection, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Understanding the disease and economic impact of avirulent avian paramyxovirus type 1 (APMV-1) infection in Great Britain.Epidemiology and infection · 2023Article
- Article
- Article
- Evaluating oral swab samples for PRRSV surveillance in weaning-age pigs under field conditions.Frontiers in veterinary science · 2023Article
- Use of compressed sensing to expedite high-throughput diagnostic testing for COVID-19 and beyond.PLoS computational biology · 2022Article
- Effects of swab pool size and transport medium on the detection and isolation of avian influenza viruses in ostriches.BMC veterinary research · 2022Article
- Article
- Use of compressed sensing to expedite high-throughput diagnostic testing for COVID-19 and beyond.medRxiv : the preprint server for health sciences · 2021Article
- Pooled nasopharyngeal swab collection in a single vial for the diagnosis of SARS CoV-2 infection: An effective cost saving method.Indian journal of medical microbiology · 2021Article
- Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA Extraction.BioMed research international · 2021Article
- Improvement of Sensitivity of Pooling Strategies for COVID-19.Computational and mathematical methods in medicine · 2021Article
- The outlook for diagnostic purposes of the 2019-novel coronavirus disease.Journal of cellular physiology · 2020Review
- Article
- Cloacal Swabs Are Unreliable Sources for Estimating Lower Gastro-Intestinal Tract Microbiota Membership and Structure in Broiler Chickens.Microorganisms · 2020Article
- Pooled RNA sample reverse transcriptase real time PCR assay for SARS CoV-2 infection: A reliable, faster and economical method.PloS one · 2020Article
- Prevalence of Avian Influenza A(H5) and A(H9) Viruses in Live Bird Markets, Bangladesh.Emerging infectious diseases · 2018Article
- Quantifying the effect of swab pool size on the detection of influenza A viruses in broiler chickens and its implications for surveillance.BMC veterinary research · 2018Article
- Optimal specimen collection and transport methods for the detection of avian influenza virus and Newcastle disease virus.BMC veterinary research · 2013Article
- Optimal size of sample pooling for RNA pool testing: An avant-garde for scaling up severe acute respiratory syndrome coronavirus-2 testing.Indian journal of medical microbiologyArticle
- Effectiveness of sample pooling strategies for diagnosis of SARS-CoV-2: Specimen pooling vs. RNA elutes pooling.Indian journal of medical microbiologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to determine whether pooling avian influenza (AI)-positive swabs with negative swabs has a detrimental effect on the sensitivity of AI real-time reverse transcription-polymerase chain reactions (rRT-PCRs). Cloacal and buccal swabs were sampled daily from 12 turkeys infected with A/goose/England/07(H2N2). For half the turkeys, each swab was mixed with four swabs from known AI-negative turkeys, and for the other half the swabs were tested individually. Bayesian modelling was used to (i) determine whether pooling the positive swabs compromised the cycle threshold (C(t)) value obtained from the rRT-PCRs, and (ii) estimate the likelihood of detection of an H2N2 infected turkey flock via rRT-PCR for pooled and individually tested swabs (cloacal and buccal) vs. the number of days post-infection of the flock. Results indicated that there was no significant effect of compromising AI rRT-PCR sensitivity by pooling a weak positive swab with negative swabs on the Ct values which were obtained. Pooled sampling was able to widen the detection window compared to individual sampling, for the same number of rRT-PCR tests. This indicates that pooled sampling would be an effective method of reducing the number of tests to be performed to determine flock status during an AI outbreak and for surveillance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.