ArticleMicrobial cell factories2012
Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Engineering Saccharomyces cerevisiae to improve heterologous abscisic acid production.Microbial cell factories · 2026Article
- Push-pull-block framework for lycopene biosynthesis: a comprehensive review of metabolic engineering strategies inFrontiers in bioengineering and biotechnology · 2026Review
- The Power of Yeast.Yeast (Chichester, England) · 2025Review
- Engineering Sanghuangporus sanghuang for enhanced (-)-aristolone production via metabolic pathway optimization and terpene synthase engineering.Applied microbiology and biotechnology · 2025Article
- Engineering cellular dephosphorylation boosts (+)-borneol production in yeast.Acta pharmaceutica Sinica. B · 2025Article
- Metabolic engineering of Saccharomyces cerevisiae for enhanced taxadiene production.Microbial cell factories · 2024Article
- Biological Properties of Sandalwood Oil and Microbial Synthesis of Its Major Sesquiterpenoids.Biomolecules · 2024Review
- Autotrophic yeast.Nature communications · 2024Article
- Glucose 6-phosphate dehydrogenase variants increase NADPH pools for yeast isoprenoid production.FEBS open bio · 2024Article
- Profiling proteomic responses to hexokinase-II depletion in terpene-producingEngineering microbiology · 2023Article
- Current advances for omics-guided process optimization of microbial manufacturing.Bioresources and bioprocessing · 2023Review
- A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast.Nature communications · 2023Review
- Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon.Microbial biotechnology · 2023Article
- Rationally engineering santalene synthase to readjust the component ratio of sandalwood oil.Nature communications · 2022Article
- Evolving Small-Molecule Biosensors with Improved Performance and Reprogrammed Ligand Preference Using OrthoRep.ACS synthetic biology · 2021Article
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
- Plasticity engineering of plant monoterpene synthases and application for microbial production of monoterpenoids.Biotechnology for biofuels · 2021Review
- Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Artificial Transcription Factors for Tuneable Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- Combined Biosynthetic Pathway Engineering and Storage Pool Expansion for High-Level Production of Ergosterol in IndustrialFrontiers in bioengineering and biotechnology · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-santalene is a precursor of sesquiterpenes with relevant commercial applications. Here, we construct an efficient Saccharomyces cerevisiae cell factory for α-santalene production.
resultsA multistep metabolic engineering strategy targeted to increase precursor and cofactor supply was employed to manipulate the yeast metabolic network in order to redirect carbon toward the desired product. To do so, genetic modifications were introduced acting to optimize the farnesyl diphosphate branch point, modulate the mevalonate pathway, modify the ammonium assimilation pathway and enhance the activity of a transcriptional activator. The approach employed resulted in an overall α-santalene yield of a 0.0052 Cmmol (Cmmol glucose)(-1) corresponding to a 4-fold improvement over the reference strain. This strategy, combined with a specifically developed continuous fermentation process, led to a final α-santalene productivity of 0.036 Cmmol (g biomass)(-1) h(-1).
conclusionsThe results reported in this work illustrate how the combination of a metabolic engineering strategy with fermentation technology optimization can be used to obtain significant amounts of the high-value sesquiterpene α-santalene. This represents a starting point toward the construction of a yeast "sesquiterpene factory" and for the development of an economically viable bio-based process that has the potential to replace the current production methods.
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