ArticlePloS one2012
Transcriptional profiling of midgut immunity response and degeneration in the wandering silkworm, Bombyx mori.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 62 citations in OpenAlex.
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- A CTL - Lys immune function maintains insect metamorphosis by preventing gut bacterial dysbiosis and limiting opportunistic infections.BMC biology · 2024Article
- Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust.Frontiers in physiology · 2023Article
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- Peptide Hormones in the Insect Midgut.Frontiers in physiology · 2020Review
- The legacy of larval infection on immunological dynamics over metamorphosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Immune gene regulation in the gut during metamorphosis in a holo- versus a hemimetabolous insect.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borerPeerJ · 2018Article
- Differentiation of lepidoptera scale cells from epidermal stem cells followed by ecdysone-regulated DNA duplication and scale secreting.Cell cycle (Georgetown, Tex.) · 2017Article
- The orange spotted cockroach (Proceedings of the West Virginia Academy of Science · 2017Article
- Analysis of gene expression in the midgut of Bombyx mori during the larval molting stage.BMC genomics · 2016Article
- Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains.PloS one · 2016Article
- Transcriptomic Analysis of Resistant and Susceptible Bombyx mori Strains Following BmNPV Infection Provides Insights into the Antiviral Mechanisms.International journal of genomics · 2016Article
- Sugarcane giant borer transcriptome analysis and identification of genes related to digestion.PloS one · 2015Article
- Functional analysis of insect molting fluid proteins on the protection and regulation of ecdysis.The Journal of biological chemistry · 2014Article
- Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.Molecular medicine reports · 2014Article
- Transcriptomic analysis of differentially expressed genes in the Ras1(CA)-overexpressed and wildtype posterior silk glands.BMC genomics · 2014Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLepidoptera insects have a novel development process comprising several metamorphic stages during their life cycle compared with vertebrate animals. Unlike most Lepidoptera insects that live on nectar during the adult stage, the Bombyx mori silkworm adults do not eat anything and die after egg-laying. In addition, the midguts of Lepidoptera insects produce antimicrobial proteins during the wandering stage when the larval tissues undergo numerous changes. The exact mechanisms responsible for these phenomena remain unclear. PRINCIPAL
findingsWe used the silkworm as a model and performed genome-wide transcriptional profiling of the midgut between the feeding stage and the wandering stage. Many genes concerned with metabolism, digestion, and ion and small molecule transportation were down-regulated during the wandering stage, indicating that the wandering stage midgut loses its normal functions. Microarray profiling, qRT-PCR and western blot proved the production of antimicrobial proteins (peptides) in the midgut during the wandering stage. Different genes of the immune deficiency (Imd) pathway were up-regulated during the wandering stage. However, some key genes belonging to the Toll pathway showed no change in their transcription levels. Unlike butterfly (Pachliopta aristolochiae), the midgut of silkworm moth has a layer of cells, indicating that the development of midgut since the wandering stage is not usual. Cell division in the midgut was observed only for a short time during the wandering stage. However, there was extensive cell apoptosis before pupation. The imbalance of cell division and apoptosis probably drives the continuous degeneration of the midgut in the silkworm since the wandering stage.
conclusionsThis study provided an insight into the mechanism of the degeneration of the silkworm midgut and the production of innate immunity-related proteins during the wandering stage. The imbalance of cell division and apoptosis induces irreversible degeneration of the midgut. The Imd pathway probably regulates the production of antimicrobial peptides in the midgut during the wandering stage.
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