ArticleThe Journal of biological chemistry2012
Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- A dual-host regulatory hub integrating biofilm formation and innate immunity resistance.Nature communications · 2026Article
- Regulatory Roles of a Novel MarR-like Protein Lmo0840 in Tetracycline-Associated Growth Phenotype and Virulence ofMicroorganisms · 2026Article
- Characterization of YhcN in stress adaptation and its complex transcriptional regulation by SlyA inFrontiers in cellular and infection microbiology · 2026Article
- The cycle of infectious fever - how it affects bacterial infections.Frontiers in cellular and infection microbiology · 2026Review
- Interrelation between Stress Management and Secretion Systems ofPathogens (Basel, Switzerland) · 2022Article
- The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.Virulence · 2019Article
- Induction of the immunoprotective coat of Yersinia pestis at body temperature is mediated by the Caf1R transcription factor.BMC microbiology · 2019Article
- Article
- An EmrB multidrug efflux pump inThe Journal of biological chemistry · 2019Article
- One for All, but Not All for One: Social Behavior during Bacterial Diseases.Trends in microbiology · 2019Review
- Biophysical and structural characterization of a zinc-responsive repressor of the MarR superfamily.PloS one · 2019Article
- Annotation of a hypothetical protein (WP_002969292.1) from Brucella abortus.Bioinformation · 2019Article
- Tuning site-specific dynamics to drive allosteric activation in a pneumococcal zinc uptake regulator.eLife · 2018Article
- AllFrontiers in cellular and infection microbiology · 2018Review
- A Precise Temperature-Responsive Bistable Switch Controlling Yersinia Virulence.PLoS pathogens · 2016Article
- Bacterial Stress Responses during Host Infection.Cell host & microbe · 2016Review
- 'Add, stir and reduce': Yersinia spp. as model bacteria for pathogen evolution.Nature reviews. Microbiology · 2016Review
- Environmental Regulation of Yersinia Pathophysiology.Frontiers in cellular and infection microbiology · 2016Review
- Regulatory principles governing Salmonella and Yersinia virulence.Frontiers in microbiology · 2015Review
- Ligand-binding pocket bridges DNA-binding and dimerization domains of the urate-responsive MarR homologue MftR from Burkholderia thailandensis.Biochemistry · 2014Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogens often rely on thermosensing to adjust virulence gene expression. In yersiniae, important virulence-associated traits are under the control of the master regulator RovA, which uses a built-in thermosensor to control its activity. Thermal upshifts encountered upon host entry induce conformational changes in the RovA dimer that attenuate DNA binding and render the protein more susceptible to proteolysis. Here, we report the crystal structure of RovA in the free and DNA-bound forms and provide evidence that thermo-induced loss of RovA activity is promoted mainly by a thermosensing loop in the dimerization domain and residues in the adjacent C-terminal helix. These determinants allow partial unfolding of the regulator upon an upshift to 37 °C. This structural distortion is transmitted to the flexible DNA-binding domain of RovA. RovA contacts mainly the DNA backbone in a low-affinity binding mode, which allows the immediate release of RovA from its operator sites. We also show that SlyA, a close homolog of RovA from Salmonella with a very similar structure, is not a thermosensor and remains active and stable at 37 °C. Strikingly, changes in only three amino acids, reflecting evolutionary replacements in SlyA, result in a complete loss of the thermosensing properties of RovA and prevent degradation. In conclusion, only minor alterations can transform a thermotolerant regulator into a thermosensor that allows adjustment of virulence and fitness determinants to their thermal environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.