ArticlePlanta2012
Overexpression of tobacco osmotin (Tbosm) in soybean conferred resistance to salinity stress and fungal infections.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 90 citations in OpenAlex.
- Genome-Wide Identification and Characterization of Thaumatin-Like Proteins in Potato (Solanum tuberosum L.) and Their Role in Stress Tolerance.Molecular biotechnology · 2026Article
- Genome-Wide Identification and Expression Analysis of the Thaumatin-like Protein Genes inCurrent issues in molecular biology · 2026Article
- Salt Tolerance in Soybean (Plants (Basel, Switzerland) · 2025Review
- Natural SNP Variation in GbOSM1 Promotor Enhances Verticillium Wilt Resistance in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Molecular analysis of the reactions inFrontiers in plant science · 2024Article
- Genetic transformation of legumes: an update.Plant cell reports · 2021Review
- ArabidopsisInternational journal of molecular sciences · 2021Article
- Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism.Plants (Basel, Switzerland) · 2021Article
- Article
- Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants.Plants (Basel, Switzerland) · 2020Review
- Transcriptional regulators involved in responses to volatile organic compounds in plants.The Journal of biological chemistry · 2019Article
- Pea p68, a DEAD-box helicase, enhances salt tolerance in marker-free transgenic plants of soybean [3 Biotech · 2019Article
- Sodium Selenate Treatment Using a Combination of Seed Priming and Foliar Spray Alleviates Salinity Stress in Rice.Frontiers in plant science · 2019Article
- Recombinant expression of osmotin in barley improves stress resistance and food safety during adverse growing conditions.PloS one · 2019Article
- Containment evaluation, cold tolerance and toxicity analysis in3 Biotech · 2018Article
- Salinity Tolerance Mechanism of Osmotin and Osmotin-like Proteins: A Promising Candidate for Enhancing Plant Salt Tolerance.Current genomics · 2017Review
- Molecular Cloning and Expression of Osmotin in a Baculovirus-Insect System: Purified Osmotin Mitigates Amyloid-beta Deposition in Neuronal Cells.Scientific reports · 2017Article
- Overexpression of a New Osmotin-Like Protein Gene (Frontiers in plant science · 2017Article
- Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato.PloS one · 2016Article
- Cloning, characterization, and bacterial over-expression of an osmotin-like protein gene from Solanum nigrum L. with antifungal activity against three necrotrophic fungi.Molecular biotechnology · 2015Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salinity and fungal diseases are the two significant constraints limiting soybean productivity. In order to address these problems, we have transformed soybean cv. Pusa 16 via somatic embryogenesis with salinity induced and apoplastically secreted pathogenesis-related tobacco osmotin (Tbosm) gene using Agrobacterium-mediated genetic transformation. Integration of Tbosm in randomly selected five GUS assay-positive independently transformed soybean plants was confirmed by polymerase chain reaction (PCR) and Southern hybridization. Reverse transcriptase-PCR (RT-PCR) and Western blotting confirmed that the Tbosm was expressed in three of the five transformed soybean plants. Further the Western blotting revealed that the truncated osmotin protein accumulated more in apoplastic fluid. The transformed (T(1)) soybean plants survived up to 200 mM NaCl, whereas non-transformed (NT) plants could withstand till 100 mM and perished at 150 mM NaCl. The biochemical analysis revealed the T(1) soybean plants accumulated higher amount of proline, chlorophyll, APX, CAT, SOD, DHAR, MDHAR, and RWC than NT plants. Leaf gas exchange measurements revealed that T(1) soybean plants maintained higher net photosynthetic rate, CO(2) assimilation, and stomatal conductance than NT plants. The three T(1) soybean plants expressing the osmotin gene also showed resistance against three important fungal pathogens of soybean--Microsphaera diffusa, Septoria glycines and Phakopsora pachyrhizi. The T(1) soybean plants produced 32-35 soybean pods/plant containing 10.3-12.0 g of seeds at 200 mM NaCl, whereas NT plant produced 28.6 soybean pods containing 9.6 g of seeds at 100 mM NaCl. The present investigation clearly shows that expression of Tbosm enhances salinity tolerance and fungal disease resistance in transformed soybean plants.
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