ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2012
Cocaine- and amphetamine-regulated transcript is the neurotransmitter regulating the action of cholecystokinin and leptin on short-term satiety in rats.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- The physiological control of eating: signals, neurons, and networks.Physiological reviews · 2022 · on this mapArticle
- Review
- Past, present and future of cocaine- and amphetamine-regulated transcript peptide.Physiology & behavior · 2021Article
- Signaling in rat brainstem via Gpr160 is required for the anorexigenic and antidipsogenic actions of cocaine- and amphetamine-regulated transcript peptide.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- Satiety induced by bile acids is mediated via vagal afferent pathways.JCI insight · 2020Article
- Blunted Vagal Cocaine- and Amphetamine-Regulated Transcript Promotes Hyperphagia and Weight Gain.Cell reports · 2020Article
- Dissecting the Role of Subtypes of Gastrointestinal Vagal Afferents.Frontiers in physiology · 2020Review
- The metabolic role of vagal afferent innervation.Nature reviews. Gastroenterology & hepatology · 2018Review
- Plasticity of vagal afferent signaling in the gut.Medicina (Kaunas, Lithuania) · 2017Review
- KATP channels in the nodose ganglia mediate the orexigenic actions of ghrelin.The Journal of physiology · 2015Article
- Deletion of leptin signaling in vagal afferent neurons results in hyperphagia and obesity.Molecular metabolism · 2014Article
- Putative roles of neuropeptides in vagal afferent signaling.Physiology & behavior · 2014Review
- CART in the regulation of appetite and energy homeostasis.Frontiers in neuroscience · 2014Review
- Article
Corrections and comments
- Retraction · 2023-01-17Falsification/Fabrication of Data · Falsification/Fabrication of Image · Investigation by Company/Institution ·
- Retracted
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Vagal CCK-A receptors (CCKARs) and leptin receptors (LRbs) interact synergistically to mediate short-term satiety. Cocaine- and amphetamine-regulated transcript (CART) peptide is expressed by vagal afferent neurons. We sought to demonstrate that this neurotransmitter regulates CCK and leptin actions on short-term satiety. We also examined the signal transduction pathways responsible for mediating the CART release from the nodose ganglia (NG). ELISA studies coupled with gene silencing of NG neurons by RNA interference elucidated intracellular signaling pathways responsible for CCK/leptin-stimulated CART release. Feeding studies followed by gene silencing of CART in NG established the role of CART in mediating short-term satiety. Immunohistochemistry was performed on rat NG neurons to confirm colocalization of CCKARs and LRbs; 63% of these neurons contained CART. Coadministration of CCK-8 and leptin caused a 2.2-fold increase in CART release that was inhibited by CCK-OPE, a low-affinity CCKAR antagonist. Transfection of cultured NG neurons with steroid receptor coactivator (SRC) or phosphatidylinositol 3-kinase (PI3K) small-interfering RNA (siRNA) or STAT3 lentiviral short hairpin RNA inhibited CCK/leptin-stimulated CART release. Silencing the expression of the EGR-1 gene inhibited the CCK/leptin-stimulated CART release but had no effect on CCK/leptin-stimulated neuronal firing. Electroporation of NG with CART siRNA inhibited CCK/leptin stimulated c-Fos expression in rat hypothalamus. Feeding studies following electroporation of the NG with CART or STAT3 siRNA abolished the effects of CCK/leptin on short-term satiety. We conclude that the synergistic interaction of low-affinity vagal CCKARs and LRbs mediates CART release from the NG, and CART is the principal neurotransmitter mediating short-term satiety. CART release from the NG involves interaction between CCK/SRC/PI3K cascades and leptin/JAK2/PI3K/STAT3 signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.