ArticleJapanese journal of ophthalmology2012
Broad-range real-time PCR assay for detection of bacterial DNA in ocular samples from infectious endophthalmitis.
Article in Japanese journal of ophthalmology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Contribution of 16S rRNA gene PCR to microbiological diagnosis of endophthalmitis: a retrospective clinical cohort study of 71 vitreous and aqueous humor samples.Journal of ophthalmic inflammation and infection · 2026Article
- Enhanced Detection of Bacterial Ocular Pathogens: A Comparative Study of Broad-Range Real-Time PCR and Conventional Culture Methods.Diagnostics (Basel, Switzerland) · 2025Article
- Detection of Mycoplasma Contamination in Transplanted Retinal Cells by Rapid and Sensitive Polymerase Chain Reaction Test.International journal of molecular sciences · 2021Article
- Clinical features of uveitis in elderly patients in central Tokyo (2013-2018).International ophthalmology · 2021Article
- The cereus matter of Bacillus endophthalmitis.Experimental eye research · 2020Review
- Epidemiology of uveitis (2013-2015) and changes in the patterns of uveitis (2004-2015) in the central Tokyo area: a retrospective study.BMC ophthalmology · 2018Article
- High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow.Stem cell research & therapy · 2018Article
- Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution.Scientific reports · 2017Article
- Development of a degenerated TaqMan real-time Q-PCR for detection of bacteria-free DNA in dialysis fluid.Biotechnology and applied biochemistry · 2017Article
- Characteristics of cases needing advanced treatment for intractable Posner-Schlossman syndrome.BMC ophthalmology · 2017Article
- Uveitis incidence in Jichi Medical University Hospital, Japan, during 2011-2015.Clinical ophthalmology (Auckland, N.Z.) · 2017Article
- A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases.Japanese journal of ophthalmology · 2017Review
- PCR detection and identification of bacterial contaminants in ocular samples from post-operative endophthalmitis.Journal of clinical and diagnostic research : JCDR · 2015Article
- Identification of torque teno virus in culture-negative endophthalmitis by representational deep DNA sequencing.Ophthalmology · 2015Observational
- Endophthalmitis: state of the art.Clinical ophthalmology (Auckland, N.Z.) · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo evaluate a broad-range real-time polymerase chain reaction (PCR) targeting the bacterial 16S rRNA gene for detection of bacterial DNA in infectious endophthalmitis.
methodsThe bacterial 16S rRNA gene was measured by quantitative real-time PCR. For the assay, bacterial DNA was prepared from 12 Gram-positive and 4 Gram-negative strains. To determine the optimum method for DNA extraction, four extraction procedures were selected by using DNA extraction program cards with and without the use of lysozyme. To evaluate PCR sensitivity, PCR fragments were amplified from Staphylococcus aureus and Escherichia coli DNA.
resultsDNA extraction using the Bacteria card(®) without enzymes resulted in detection of all the tested strains at concentrations ≥ 10(7) copies/mL. Extraction with the Bacteria card(®) with lysozyme resulted in detection of all the tested strains at concentrations ≥ 10(6) copies/mL, indicative of no significant difference between the two procedures. DNA extraction using the Virus card(®), both with and without enzymes, resulted in reduced efficiency of detection of all strains compared with use of the Bacteria card(®). The PCR could detect as few as 1-10 colony-forming units (CFU) in diluted vitreous samples per reaction, and all tested bacterial species known to cause endophthalmitis were detected.
conclusionsBacterial 16S-specific PCR can comprehensively detect the main causative bacteria of clinically suspected endophthalmitis.
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