ArticleOsteoarthritis and cartilage2012
In vivo reduction or blockade of interleukin-1β in primary osteoarthritis influences expression of mediators implicated in pathogenesis.
Article in Osteoarthritis and cartilage, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
71 citing papers in PubMed, 2 syntheses or guidelines pooled it, 147 citations in OpenAlex.
- Naturally Occurring Osteoarthritis Features and Treatments: Systematic Review on the Aged Guinea Pig Model.International journal of molecular sciences · 2022Pooled it
- Associations between adipokines gene polymorphisms and knee osteoarthritis: a meta-analysis.BMC musculoskeletal disorders · 2022Pooled it
- Radiological and biochemical effects (CTX-II, MMP-3, 8, and 13) of low-level laser therapy (LLLT) in chronic osteoarthritis in Al-Kharj, Saudi Arabia.Lasers in medical science · 2017Trial
- Identifying Plasma Biomarkers That Predict Patient-Reported Outcomes Following Treatment for Trapeziometacarpal Osteoarthritis Using Machine Learning.International journal of molecular sciences · 2025Article
- Pulchinenoside C Attenuates the Development of Osteoarthritis by Inhibiting the PI3K/AKT/NF-κB Signalling Pathway.Journal of cellular and molecular medicine · 2025Article
- Pulsed electromagnetic field ameliorates the progression of osteoarthritis via the Sirt1/NF-κB pathway.Arthritis research & therapy · 2025Article
- Syringic Acid Attenuates the IL-1β-Induced Akt Pathway in Chondrocyte ATDC5 Cells.Combinatorial chemistry & high throughput screening · 2025Article
- Chronic intermittent hypobaric hypoxia alleviates early-stage posttraumatic osteoarthritis via NF-κB/Nrf2 pathway in mice.Journal of orthopaedic surgery and research · 2024Article
- Amentoflavone maintaining extracellular matrix homeostasis and inhibiting subchondral bone loss in osteoarthritis by inhibiting ERK, JNK and NF-κB signaling pathways.Journal of orthopaedic surgery and research · 2024Article
- Equine Musculoskeletal Pathologies: Clinical Approaches and Therapeutical Perspectives-A Review.Veterinary sciences · 2024Review
- Progress and Challenges of Topical Delivery Technologies Meditated Drug Therapy for Osteoarthritis.International journal of nanomedicine · 2024Review
- Bergamottin (Ber) ameliorates the progression of osteoarthritis via the Sirt1/NF-κB pathway.Frontiers in pharmacology · 2024Article
- Chondrocyte targeting gold nanoparticles protect growth plate against inflammatory damage by maintaining cartilage balance.Materials today. Bio · 2023Article
- A pre-market interventional, single-arm clinical investigation of a new topical lotion based on hyaluronic acid and peptides, EGYFILBMC musculoskeletal disorders · 2023Article
- Article
- Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway.Stem cell research & therapy · 2023Article
- Nesfatin-1 suppresses autophagy of chondrocytes in osteoarthritis via remodeling of cytoskeleton and inhibiting RhoA/ROCK signal pathway.Journal of orthopaedic surgery and research · 2023Article
- Human Dental Follicle Cell-Derived Small Extracellular Vesicles Attenuate Temporomandibular Joint Cartilage Damage through Inhibiting HIF-2Journal of tissue engineering and regenerative medicine · 2023Article
- An Insight on the Biomedical Potential of Portuguese Propolis from Gerês.Foods (Basel, Switzerland) · 2022Article
- Role of Mesenchymal Stem Cells and Their Paracrine Mediators in Macrophage Polarization: An Approach to Reduce Inflammation in Osteoarthritis.International journal of molecular sciences · 2022Review
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
objectiveDiminish interleukin-1β (IL-1β) signaling in a model of primary osteoarthritis by RNA interference-based transcript reduction or receptor blockade, and quantify changes incurred on transcript expression of additional mediators.
methodsKnees of Hartley guinea pigs were collected at 120 and 180 days of age following injection with viral vectors (N = 4/treatment group/date) at 60 days. Two groups received either adeno-associated viral serotype 5 vector containing a knockdown sequence (TV), or adenoviral vector encoding for IL-1 receptor antagonist protein (Ad-IRAP); treatments were contrasted with opposite knees administered corresponding vector controls. A third group evaluated TV relative to saline-only injected knees. Chondropathy and immunohistochemistry findings were compared to untreated guinea pigs. Transcript expression levels in cartilage were calculated using the comparative CT (2(-ΔΔCT)) method and analyzed by one-way analysis of variance (ANOVA) with pairwise comparisons using Tukey 95% confidence intervals.
resultsVector transduction was confirmed at both harvest dates. TV and Ad-IRAP, relative to vector controls, significantly decreased IL-1β. Inflammatory mediators [tumor necrosis factor-α (TNF-α), IL-8, interferon-γ (IFN-γ)], and catabolic matrix metalloproteinase 13 (MMP13) were also decreased, while anabolic transforming growth factor-β1 (TGF-β1) was increased. IL-1β was also decreased by TV vs saline, with a decrease in MMP13 and increase TGF-β1; TNF-α, IL-8, and IFN-γ were transiently increased.
conclusionsThis work confirmed that a reduction in IL-1β signaling was accomplished by either method, resulting in decreased expression of three inflammatory mediators and one catabolic agent, and increased expression of an anabolic molecule. Thus, evidence is provided that IL-1β serves a role in vivo in spontaneous osteoarthritis and that these translational tools may provide beneficial disease modification.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.