ArticleMolecular microbiology2012
Inhibitory mechanism of the Qβ lysis protein A2.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 18 citations in OpenAlex.
- Phage-derived proteins and conjugates induce pilus detachment.Journal of bacteriology · 2026Article
- Carboxyl-Terminal Region of the Maturation Protein of RNA Coliphage Qβ Mediates Host Attachment, Virion Formation, and Cell Lysis.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Engineered Proteins Mimic ssRNA Phage to Disrupt Type IV Pili.bioRxiv : the preprint server for biology · 2025Article
- Physiological characterization of single-gene lysis proteins.Journal of bacteriology · 2024Article
- Physiological characterization of single gene lysis proteins.bioRxiv : the preprint server for biology · 2023Article
- Review
- The balance between fitness advantages and costs drives adaptation of bacteriophage Qβ to changes in host density at different temperatures.Frontiers in microbiology · 2023Article
- YdfD, a Lysis Protein of the Qin Prophage, Is a Specific Inhibitor of the IspG-Catalyzed Step in the MEP Pathway ofInternational journal of molecular sciences · 2022Article
- Review
- Isolation, Genomic Analysis, and Preliminary Application of a BovineFrontiers in veterinary science · 2021Article
- Single-gene lysis in the metagenomic era.Current opinion in microbiology · 2020Review
- Phage single-gene lysis: Finding the weak spot in the bacterial cell wall.The Journal of biological chemistry · 2019Review
- Function of the RNA Coliphage Qβ Proteins in Medical In Vitro Evolution.Methods and protocols · 2018Review
- Structures of Qβ virions, virus-like particles, and the Qβ-MurA complex reveal internal coat proteins and the mechanism of host lysis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Bacteriophage therapy against Enterobacteriaceae.Virologica Sinica · 2015Review
- A(2) expression and assembly regulates lysis in Qβ infections.Microbiology (Reading, England) · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The lysis protein A2 , present as a single copy on the surface of Qβ virion particles, was previously shown to inhibit the activity of MurA, an enzyme that catalyses the first committed step of murein biosynthesis. Here we report experiments with a two-hybrid study that indicates A2 and MurA interact directly. Moreover, experiments with a soluble MBP-A2 fusion indicate that the interaction between MurA and A2 is dependent on a substrate-induced conformational change featured in the UDP-NAG-liganded state of MurA but not the tetrahedral intermediate state. Moreover, based on the location of L138Q, the original dominant A2 -resistant mutant that identified MurA as the target, a directed mutagenesis strategy has identified a continuous surface required for A2 binding. This surface spans the catalytic loop/cleft and encompasses both the catalytic and C-terminal domains. These data support a model in which A2 preferentially binds MurA liganded with UDP-NAG, thereby preventing catalysis by occluding PEP from accessing the active site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.