ArticleInterdisciplinary perspectives on infectious diseases2012
Adaptation of Ritchie's Method for Parasites Diagnosing with Minimization of Chemical Products.
Article in Interdisciplinary perspectives on infectious diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 21 citations in OpenAlex.
- Comparative evaluation of Midi ParasepParasites & vectors · 2026Article
- Climatic and Host-Related Drivers of Gastrointestinal Parasite Dynamics in Domestic Ruminants of North Bengal, India.Animals : an open access journal from MDPI · 2026Article
- Improvement of the Diagnostic Method for the Detection of Parasite Eggs Using the Formalin Ethyl Acetate Concentration Technique.Acta parasitologica · 2024Article
- Systematic Review of Diagnostic Approaches for Human Giardiasis: Unveiling Optimal Strategies.Diagnostics (Basel, Switzerland) · 2024Review
- Micronutrients and socio-demographic factors were major predictors of anaemia among the Ethiopian population.The British journal of nutrition · 2023Article
- Prevalence, intensity and associated risk factors of Schistosoma mansoni infections among schoolchildren around Lake Tana, northwestern Ethiopia.PLoS neglected tropical diseases · 2021Article
- Locally acquired infection with Dibothriocephalus nihonkaiense (=Diphyllobothrium nihonkaiense) in France: the importance of molecular diagnosis.Parasitology research · 2020Article
- Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples.Molecular biology reports · 2020Article
- Formalin-ethyl acetate concentration, FLOTAC Pellet and anal swab techniques for the diagnosis of intestinal parasites.Parasitology research · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Latin America, Africa, and Asia present wide dissemination and high prevalence rates of waterborne parasitic diseases, which is a strong indicative of the fragility of public sanitation systems. In this context, parasitological analyses represent extremely relevant instruments. Several parasite diagnosis methods exist, among which Ritchie's method (1948) stands out. This method uses formaldehyde and ether, two reagents of toxicological importance that can cause damages to environmental and occupational health. The present study aimed to compare Ritchie's method modified by Régis Anécimo, without use of solvents, with the traditional Ritchie's method, routinely used for helminth and protozoa diagnosing in Brazil. Some changes were introduced in the modified method, such as controlled increase of water temperature used after stool dilution and substitution of formaldehyde and ether by a neutral detergent before material centrifugation for observation of parasites. In examined samples by both methods, multiple infections were commonly observed; the modified method presented a similar sensitivity to identify the parasites. The development of analytic diagnosis methods that minimize the use of chemical products like ether and formaldehyde represents an important tool to prevent occupational diseases among exposed professionals, as well as to preserve environmental quality through the use of clean techniques.
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