ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Deletion of the presynaptic scaffold CAST reduces active zone size in rod photoreceptors and impairs visual processing.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 99 citations in OpenAlex.
- Article
- A non-conducting role of the CaeLife · 2024Article
- Presynaptic Proteins and Their Roles in Visual Processing by the Retina.Annual review of vision science · 2024Review
- A non-conducting role of the CabioRxiv : the preprint server for biology · 2024Article
- The Structural and Functional Integrity of Rod Photoreceptor Ribbon Synapses Depends on Redundant Actions of Dynamins 1 and 3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Structure, Function, and Molecular Landscapes of the Aging Retina.Annual review of vision science · 2023Review
- Critical Role of the Presynaptic Protein CAST in Maintaining the Photoreceptor Ribbon Synapse Triad.International journal of molecular sciences · 2023Article
- The first synapse in vision in the aging mouse retina.Frontiers in cellular neuroscience · 2023Article
- Post-developmental plasticity of the primary rod pathway allows restoration of visually guided behaviors.Current biology : CB · 2022Article
- Mechanisms of Synaptic Vesicle Exo- and Endocytosis.Biomedicines · 2022Review
- Article
- Stable and Flexible Synaptic Transmission Controlled by the Active Zone Protein Interactions.International journal of molecular sciences · 2021Review
- Article
- Transmission at rod and cone ribbon synapses in the retina.Pflugers Archiv : European journal of physiology · 2021Review
- Development and maintenance of vision's first synapse.Developmental biology · 2021Review
- Synaptic Remodeling in the Cone Pathway After Early Postnatal Horizontal Cell Ablation.Frontiers in cellular neuroscience · 2021Article
- Neurotransmitter Release Site Replenishment and Presynaptic Plasticity.International journal of molecular sciences · 2020Review
- Disturbed Presynaptic CaiScience · 2020Article
- Presynaptic development is controlled by the core active zone proteins CAST/ELKS.The Journal of physiology · 2020Article
- Molecular mechanisms underlying selective synapse formation of vertebrate retinal photoreceptor cells.Cellular and molecular life sciences : CMLS · 2020Review
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How size and shape of presynaptic active zones are regulated at the molecular level has remained elusive. Here we provide insight from studying rod photoreceptor ribbon-type active zones after disruption of CAST/ERC2, one of the cytomatrix of the active zone (CAZ) proteins. Rod photoreceptors were present in normal numbers, and the a-wave of the electroretinogram (ERG)--reflecting their physiological population response--was unchanged in CAST knock-out (CAST(-/-)) mice. Using immunofluorescence and electron microscopy, we found that the size of the rod presynaptic active zones, their Ca(2+) channel complement, and the extension of the outer plexiform layer were diminished. Moreover, we observed sprouting of horizontal and bipolar cells toward the outer nuclear layer indicating impaired rod transmitter release. However, rod synapses of CAST(-/-) mice, unlike in mouse mutants for the CAZ protein Bassoon, displayed anchored ribbons, normal vesicle densities, clustered Ca(2+) channels, and essentially normal molecular organization. The reduction of the rod active zone size went along with diminished amplitudes of the b-wave in scotopic ERGs. Assuming, based on the otherwise intact synaptic structure, an unaltered function of the remaining release apparatus, we take our finding to suggest a scaling of release rate with the size of the active zone. Multielectrode-array recordings of retinal ganglion cells showed decreased contrast sensitivity. This was also observed by optometry, which, moreover, revealed reduced visual acuity. We conclude that CAST supports large active zone size and high rates of transmission at rod ribbon synapses, which are required for normal vision.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.