ArticleJournal of clinical microbiology2012
Usability and performance of CHROMagar STEC medium in detection of Shiga toxin-producing Escherichia coli strains.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 84 citations in OpenAlex.
- Whole-Genome Sequence Analysis of Shiga Toxin-ProducingMicroorganisms · 2026Article
- Genomic Markers Distinguishing Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2025Article
- Effect of conventional grain-fed and grass-fed feeding systems on fecal microbiota and shiga toxin-producing Escherichia coli in beef cattle.BMC microbiology · 2025Article
- Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels.Bioactive materials · 2021Article
- Migratory birds as a potential overseas transmitter of Shiga toxin-producingInternational journal of veterinary science and medicine · 2021Article
- Evaluation of Cattle for Naturally Colonized Shiga Toxin-ProducingInternational journal of microbiology · 2021Article
- Evaluation of chromogenic selective agar (CHROMagar STEC) for the direct detection of Shiga toxin-producingJournal of medical microbiology · 2020Article
- Prevalence and concentration of stx+ E. coli and E. coli O157 in bovine manure from Florida farms.PloS one · 2019Article
- Implications of stx loss for clinical diagnostics of Shiga toxin-producing Escherichia coli.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town.BMC microbiology · 2018Article
- Tellurite Resistance in Shiga Toxin-Producing Escherichia coli.Current microbiology · 2018Article
- Single Locked Nucleic Acid-Enhanced Nanopore Genetic Discrimination of Pathogenic Serotypes and Cancer Driver Mutations.ACS nano · 2018Article
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- Article
- A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics.Clinical microbiology reviews · 2017Review
- Development of a real-time PCR assay and comparison to CHROMagarAfrican journal of laboratory medicine · 2017Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Detection, Characterization, and Typing of Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Review
- Comparison of Six Chromogenic Agar Media for the Isolation of a Broad Variety of Non-O157 Shigatoxin-Producing Escherichia coli (STEC) Serogroups.International journal of environmental research and public health · 2015Article
- Assessing the public health risk of Shiga toxin-producing Escherichia coli by use of a rapid diagnostic screening algorithm.Journal of clinical microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The performance and usability of CHROMagar STEC medium (CHROMagar Microbiology, Paris, France) for routine detection of Shiga toxin-producing Escherichia coli (STEC) strains were examined. The ability of the medium to selectively propagate STEC strains differing by their serotypes and virulence genes was studied with a collection of diarrheagenic E. coli isolates (n = 365) consisting of 49 different serotypes and with non-STEC and other bacterial isolates (n = 264). A total of 272 diarrheagenic E. coli (75.0%) isolates covering 24 different serotypes grew on CHROMagar STEC. The highest detection sensitivities were observed within the STEC serogroups O26 (90.0%), O111 (100.0%), O121 (100.0%), O145 (100.0%), and O157 (84.9%), and growth on CHROMagar STEC was highly associated with the presence of the tellurite resistance gene (terD). The specificity of the medium was 98.9%. In addition, CHROMagar STEC was used in parallel with a Shiga toxin-detecting immunoassay (Ridaquick Verotoxin/O157 Combi; R-biopharm, Darmstadt, Germany) to screen fecal specimens (n = 47) collected from patients suffering from hemorrhagic diarrhea. Positive growth on CHROMagar STEC was confirmed by the Premier EHEC enzyme immunoassay (Meridian Bioscience, Inc., Cincinnati, OH), and discrepant results between the two screening methods were confirmed by stx gene-detecting PCR. All 16 of the 47 stool samples that showed positive growth on CHROMagar STEC were also positive in the confirmatory tests. CHROMagar STEC proved to be an interesting option for STEC screening, allowing good detection sensitivity and specificity and permitting strain isolation for further outbreak investigations when required.
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