ArticleThe Journal of biological chemistry2012
dTIS11 Protein-dependent polysomal deadenylation is the key step in AU-rich element-mediated mRNA decay in Drosophila cells.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
- Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.RNA (New York, N.Y.) · 2026Article
- mRNA decay pre-complex assembly drives timely cell-state transitions during differentiation.Cell reports · 2025Article
- Newly synthesized mRNA escapes translational repression during the acute phase of the mammalian unfolded protein response.PloS one · 2022Article
- Multilayered gene control drives timely exit from the stem cell state in uncommitted progenitors duringGenes & development · 2018Article
- ARE-mediated decay controls gene expression and cellular metabolism upon oxygen variations.Scientific reports · 2018Article
- The role of RNA-binding protein tristetraprolin in cancer and immunity.Medical oncology (Northwood, London, England) · 2017Review
- The control of inflammation via the phosphorylation and dephosphorylation of tristetraprolin: a tale of two phosphatases.Biochemical Society transactions · 2016Review
- Exaptive origins of regulated mRNA decay in eukaryotes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Oligoadenylation of 3' decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells.RNA (New York, N.Y.) · 2016Article
- RNA binding proteins as regulators of immune cell biology.Clinical and experimental immunology · 2016Review
- General and MicroRNA-Mediated mRNA Degradation Occurs on Ribosome Complexes in Drosophila Cells.Molecular and cellular biology · 2015Article
- Deadenylation of mRNA by the CCR4-NOT complex in Drosophila: molecular and developmental aspects.Frontiers in genetics · 2014Review
- Article
- Kiss your tail goodbye: the role of PARN, Nocturnin, and Angel deadenylases in mRNA biology.Biochimica et biophysica actaReview
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Authors and funding
7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The destabilization of AU-rich element (ARE)-containing mRNAs mediated by proteins of the TIS11 family is conserved among eukaryotes including Drosophila. Previous studies have demonstrated that Tristetraprolin, a human protein of the TIS11 family, induces the degradation of ARE-containing mRNAs through a large variety of mechanisms including deadenylation, decapping, and P-body targeting. We have previously shown that the degradation of the mRNA encoding the antimicrobial peptide Cecropin A1 (CecA1) is controlled by the TIS11 protein (dTIS11) in Drosophila cells. In this study, we used CecA1 mRNA as a model to investigate the molecular mechanism of dTIS11-mediated mRNA decay. We observed that during the biphasic deadenylation and decay process of this mRNA, dTIS11 enhances deadenylation performed by the CCR4-CAF-NOT complex while the mRNA is still associated with ribosomes. Sequencing of mRNA degradation intermediates revealed that the complete deadenylation of the mRNA triggers its decapping and decay in both the 5'-3' and the 3'-5' directions. Contrary to the observations made for its mammalian homologs, overexpression of dTIS11 does not promote the localization of ARE-containing mRNAs in P-bodies but rather decreases the accumulation of CecA1 mRNA in these structures by enhancing the degradation process. Therefore, our results suggest that proteins of the TIS11 family may have acquired additional functions in the course of evolution from invertebrates to mammals.
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