ArticlePlant physiology2012
Tie-dyed2 encodes a callose synthase that functions in vein development and affects symplastic trafficking within the phloem of maize leaves.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 75 citations in OpenAlex.
- Integrated genomic and transcriptomic analysis reveals the mechanisms underlying leaf variegation in 'Gonggan' mandarin.BMC plant biology · 2025Article
- Comparative Analysis of Sucrose-Regulatory Genes in High- and Low-Sucrose Sister Clones of Sugarcane.Plants (Basel, Switzerland) · 2024Article
- Genome-Wide Identification of Callose Synthase Family Genes and Their Expression Analysis in Floral Bud Development and Hormonal Responses inPlants (Basel, Switzerland) · 2023Article
- Glucan Synthase-like 2 is Required for Seed Initiation and Filling as Well as Pollen Fertility in Rice.Rice (New York, N.Y.) · 2023Article
- Contrasting Metabolisms in Green and White Leaf Sectors of VariegatedInternational journal of molecular sciences · 2023Article
- The maize callose synthaseMolecular breeding : new strategies in plant improvement · 2023Article
- PbrCalS5, a callose synthase protein, is involved in pollen tube growth in Pyrus bretschneideri.Planta · 2022Article
- Alkaline α-galactosidase 2 (CsAGA2) plays a pivotal role in mediating source-sink communication in cucumber.Plant physiology · 2022Article
- Characterization and Expression Analyses of Callose Synthase Enzyme (Cals) Family Genes in Maize (Zea mays L.).Biochemical genetics · 2022Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Article
- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Maize Brittle Stalk2-Like3, encoding a COBRA protein, functions in cell wall formation and carbohydrate partitioning.The Plant cell · 2021Article
- Review
- Diverse regulation of plasmodesmal architecture facilitates adaptation to phloem translocation.Journal of experimental botany · 2020Review
- Integrated Genome-Scale Analysis Identifies Novel Genes and Networks Underlying Senescence in Maize.The Plant cell · 2019Article
- Plasmodesmata-Related Structural and Functional Proteins: The Long Sought-After Secrets of a Cytoplasmic Channel in Plant Cell Walls.International journal of molecular sciences · 2019Review
- A Subsidiary Cell-Localized Glucose Transporter Promotes Stomatal Conductance and Photosynthesis.The Plant cell · 2019Article
- 12-Oxo-Phytodienoic Acid Acts as a Regulator of Maize Defense against Corn Leaf Aphid.Plant physiology · 2019Article
- Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.Journal of experimental botany · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tie-dyed2 (tdy2) mutant of maize (Zea mays) displays variegated green and yellow leaves. Intriguingly, the yellow leaf tissues hyperaccumulate starch and sucrose, the soluble sugar transported long distance through the phloem of veins. To determine the molecular basis for Tdy2 function, we cloned the gene and found that Tdy2 encodes a callose synthase. RNA in situ hybridizations revealed that in developing leaves, Tdy2 was most highly expressed in the vascular tissue. Comparative expression analysis with the vascular marker maize PINFORMED1a-yellow fluorescent protein confirmed that Tdy2 was expressed in developing vein tissues. To ascertain whether the defect in tdy2 leaves affected the movement of sucrose into the phloem or its long-distance transport, we performed radiolabeled and fluorescent dye tracer assays. The results showed that tdy2 yellow leaf regions were defective in phloem export but competent in long-distance transport. Furthermore, transmission electron microscopy of tdy2 yellow leaf regions showed incomplete vascular differentiation and implicated a defect in cell-to-cell solute movement between phloem companion cells and sieve elements. The disruption of sucrose movement in the phloem in tdy2 mutants provides evidence that the Tdy2 callose synthase functions in vascular maturation and that the vascular defects result in impaired symplastic trafficking into the phloem translocation stream.
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