ArticleMethods in enzymology2012
DNA methyltransferase accessibility protocol for individual templates by deep sequencing.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Methylscaper: an R/Shiny app for joint visualization of DNA methylation and nucleosome occupancy in single-molecule and single-cell data.Bioinformatics (Oxford, England) · 2021Article
- Molecular and computational approaches to map regulatory elements in 3D chromatin structure.Epigenetics & chromatin · 2021Review
- Chromatin accessibility profiling methods.Nature reviews. Methods primers · 2021Article
- Absolute nucleosome occupancy map for theGenome research · 2019Article
- High Fractional Occupancy of a Tandem Maf Recognition Element and Its Role in Long-Range β-Globin Gene Regulation.Molecular and cellular biology · 2016Article
- Human lymphoid translocation fragile zones are hypomethylated and have accessible chromatin.Molecular and cellular biology · 2015Article
- Review
- Multiplex mapping of chromatin accessibility and DNA methylation within targeted single molecules identifies epigenetic heterogeneity in neural stem cells and glioblastoma.Genome research · 2014Article
- Local depletion of DNA methylation identifies a repressive p53 regulatory region in the NEK2 promoter.The Journal of biological chemistry · 2013Article
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5 authors.
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Abstract
A single-molecule probe of chromatin structure can uncover dynamic chromatin states and rare epigenetic variants of biological importance that bulk measures of chromatin structure miss. In bisulfite genomic sequencing, each sequenced clone records the methylation status of multiple sites on an individual molecule of DNA. An exogenous DNA methyltransferase can thus be used to image nucleosomes and other protein-DNA complexes. In this chapter, we describe the adaptation of this technique, termed Methylation Accessibility Protocol for individual templates, to modern high-throughput sequencing, which both simplifies the workflow and extends its utility.
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