ArticleNucleus (Austin, Tex.)
Regulation of nuclear envelope permeability in cell death and survival.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- CabioRxiv : the preprint server for biology · 2026Article
- Brief and Diverse Excitotoxic Insults Increase the Neuronal Nuclear Membrane Permeability in the Neonatal Brain, Resulting in Neuronal Dysfunction and Cell Death.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Trauma in Neonatal Acute Brain Slices Alters Calcium and Network Dynamics and Causes Calpain-Mediated Cell Death.eNeuro · 2024Article
- Pathological tau signatures and nuclear alterations in neurons, astrocytes and microglia in Alzheimer's disease, progressive supranuclear palsy, and dementia with Lewy bodies.Brain pathology (Zurich, Switzerland) · 2023Article
- Nuclear entry and egress of parvoviruses.Molecular microbiology · 2022Review
- G2/M checkpoint regulation and apoptosis facilitate the nuclear egress of parvoviral capsids.Frontiers in cell and developmental biology · 2022Article
- Orchestration of Force Generation and Nuclear Collapse in Apoptotic Cells.International journal of molecular sciences · 2021Review
- FAM111A induces nuclear dysfunction in disease and viral restriction.EMBO reports · 2021Article
- The nuclear envelope: target and mediator of the apoptotic process.Cell death discovery · 2020Review
- Imaging of morphological and biochemical hallmarks of apoptosis with optimized optogenetic tools.The Journal of biological chemistry · 2019Article
- Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg.Scientific reports · 2019Article
- FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain ofFrontiers in neuroscience · 2019Article
- Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification.Scientific reports · 2018Article
- Targeted two-photon chemical apoptotic ablation of defined cell types in vivo.Nature communications · 2017Article
- Article
- Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia.Cellular and molecular life sciences : CMLS · 2014Article
- Bcl-2 family in inter-organelle modulation of calcium signaling; roles in bioenergetics and cell survival.Journal of bioenergetics and biomembranes · 2014Review
- Clearance of Apoptotic Bodies, NETs, and Biofilm DNA: Implications for Autoimmunity.Frontiers in immunology · 2014Review
- Non-canonical function of Bax in stress-induced nuclear protein redistribution.Cellular and molecular life sciences : CMLS · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear pore complex (NPC) mediates macromolecular exchange between nucleus and cytoplasm. It is a regulated channel whose functional properties are modulated in response to the physiological status of the cell. Identifying the factors responsible for regulating NPC activity is crucial to understand how intracellular signaling cues are integrated at the level of this channel to control nucleocytoplasmic trafficking. For proteins lacking active translocation signals the NPC acts as a molecular sieve limiting passage across the nuclear envelope (NE) to proteins with a MW below ~40 kD. Here, we investigate how this permeability barrier is altered in paradigms of cell death and cell survival, i.e., apoptosis induction via staurosporine, and enhanced viability via overexpression of Bcl-2. We monitor dynamic changes of the NPC's size-exclusion limit for passive diffusion by confocal time-lapse microscopy of cells undergoing apoptosis, and use different diffusion markers to determine how Bcl-2 expression affects steady-state NE permeability. We show that staurosporine triggers an immediate and gradual leakiness of the NE preceding the appearance of apoptotic hallmarks. Bcl-2 expression leads to a constitutive increase in NE permeability, and its localization at the NE is sufficient for the effect, evincing a functional role for Bcl-2 at the nuclear membrane. In both settings, NPC leakiness correlates with reduced Ca²⁺ in internal stores, as demonstrated by fluorometric measurements of ER/NE Ca²⁺ levels. By comparing two cellular models with opposite outcome these data pinpoint ER/NE Ca²⁺ as a general and physiologically relevant regulator of the permeability barrier function of the NPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.