ArticleBMC medical genomics2012
Structural, functional and molecular analysis of the effects of aging in the small intestine and colon of C57BL/6J mice.
Article in BMC medical genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Tissue and cellular spatiotemporal dynamics in colon aging.Nature biotechnology · 2026Article
- Probiotics reduce the severity of DSS-induced colitis in aged mice by enhancing intestinal barrier function and modulating gut microbiota.GeroScience · 2026Article
- Multitissue, multi-time point transcriptomic atlas of aging in mice and rats.Science advances · 2026Article
- New indicator of small intestinal ageing in senescence-accelerated mice.The journal of physiological sciences : JPS · 2025Article
- Fermented mixed grain ameliorates chronic stress-induced depression-like behavior and memory deficit.Food science and biotechnology · 2024Article
- Impairments of intestinal arginine and NO metabolisms trigger aging-associated intestinal barrier dysfunction and 'inflammaging'.Redox biology · 2022Article
- Review
- Uptake and Transport of Naringenin and Its Antioxidant Effects in Human Intestinal Epithelial Caco-2 Cells.Frontiers in nutrition · 2022Article
- The Aged Intestine: Performance and Rejuvenation.Aging and disease · 2021Review
- Article
- Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells.Pharmaceutics · 2020Article
- Ageing, metabolism and the intestine.EMBO reports · 2020Review
- DNA Methylation Analysis Validates Organoids as a Viable Model for Studying Human Intestinal Aging.Cellular and molecular gastroenterology and hepatology · 2020Article
- Article
- Review
- Article
- Investigating Gut Permeability in Animal Models of Disease.Frontiers in physiology · 2018Article
- Perinatal Malnutrition Leads to Sexually Dimorphic Behavioral Responses with Associated Epigenetic Changes in the Mouse Brain.Scientific reports · 2017Article
- The TNF-α antagonist etanercept reverses age-related decreases in colonic SERT expression and faecal output in mice.Scientific reports · 2017Article
- Identification of the principal transcriptional regulators for low-fat and high-fat meal responsive genes in small intestine.Nutrition & metabolism · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBy regulating digestion and absorption of nutrients and providing a barrier against the external environment the intestine provides a crucial contribution to the maintenance of health. To what extent aging-related changes in the intestinal system contribute to the functional decline associated with aging is still under debate.
methodsYoung (4 M) and old (21 M) male C57BL/6J mice were fed a control low-fat (10E%) or a high-fat diet (45E%) for 2 weeks. During the intervention gross energy intake and energy excretion in the feces were measured. After sacrifice the small and large intestine were isolated and the small intestine was divided in three equal parts. Swiss rolls were prepared of each of the isolated segments for histological analysis and the luminal content was isolated to examine alterations in the microflora with 16S rRNA Q-PCR. Furthermore, mucosal scrapings were isolated from each segment to determine differential gene expression by microarray analysis and global DNA methylation by pyrosequencing.
resultsDigestible energy intake was similar between the two age groups on both the control and the high-fat diet. Microarray analysis on RNA from intestinal scrapings showed no marked changes in expression of genes involved in metabolic processes. Decreased expression of Cubilin was observed in the intestine of 21-month-old mice, which might contribute to aging-induced vitamin B12 deficiency. Furthermore, microarray data analysis revealed enhanced expression of a large number of genes involved in immune response and inflammation in the colon, but not in the small intestine of the 21-month-old mice. Aging-induced global hypomethylation was observed in the colon and the distal part of the small intestine, but not in the first two sections of the small intestine.
conclusionIn 21-month old mice the most pronounced effects of aging were observed in the colon, whereas very few changes were observed in the small intestine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.