ArticleJournal of neurochemistry2012
Differential neurite outgrowth is required for axon specification by cultured hippocampal neurons.
Article in Journal of neurochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 58 citations in OpenAlex.
- Molecular and Cellular Mechanisms of Synaptogenesis and Synaptic Refinement During Cerebellar Circuit Formation.International journal of molecular sciences · 2026Review
- Article
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Constructive Neuroengineering of Axon Polarization Control Using Modifiable Agarose Gel Platforms for Neuronal Circuit Construction.Gels (Basel, Switzerland) · 2025Article
- Pip5k1γ regulates axon formation by limiting Rap1 activity.Life science alliance · 2024Article
- Article
- Sevoflurane exposure during the second trimester induces neurotoxicity in offspring rats by hyperactivation of PARP-1.Psychopharmacology · 2022Article
- NgCAM and VAMP2 reveal that direct delivery and dendritic degradation maintain axonal polarity.Molecular biology of the cell · 2022Article
- Hepatocyte Growth Factor-Preconditioned Neural Progenitor Cells Attenuate Astrocyte Reactivity and Promote Neurite Outgrowth.Frontiers in cellular neuroscience · 2021Article
- A cross-platform approach to characterize and screen potential neurovascular unit toxicants.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- Review
- Effect of Laminin Derived Peptides IKVAV and LRE Tethered to Hyaluronic Acid on hiPSC Derived Neural Stem Cell Morphology, Attachment and Neurite Extension.Journal of functional biomaterials · 2020Article
- βPix-d promotes tubulin acetylation and neurite outgrowth through a PAK/Stathmin1 signaling pathway.PloS one · 2020Article
- Linking Cell Polarity to Cortical Development and Malformations.Frontiers in cellular neuroscience · 2019Review
- Secretome of Differentiated PC12 Cells Enhances Neuronal Differentiation in Human Mesenchymal Stem Cells Via NGF-Like Mechanism.Molecular neurobiology · 2018Article
- Controlled Arrangement of Neuronal Cells on Surfaces Functionalized with Micropatterned Polymer Brushes.ACS omega · 2018Article
- Dependence and Homeostasis of Membrane Impedance on Cell Morphology in Cultured Hippocampal Neurons.Scientific reports · 2018Article
- Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation.Micromachines · 2018Article
- The Development of Neuronal Polarity: A Retrospective View.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Neurons with Multiple Axons Have Functional Axon Initial Segments.Neuroscience bulletin · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Formation of an axon is the first morphological evidence of neuronal polarization, visible as a profound outgrowth of the axon compared with sibling neurites. One unsolved question on the mechanism of axon formation is the role of axon outgrowth in axon specification. This question was difficult to assess, because neurons freely extend their neurites in a conventional culture. Here, we leveraged surface nano/micro-modification techniques to fabricate a template substrate for constraining neurite lengths of cultured neurons. Using the template, we asked (i) Do neurons polarize even if all neurites cannot grow sufficiently long? (ii) Would the neurite be fated to become an axon if only one was allowed to grow long? A pattern with symmetrical short paths (20 μm) was used to address the former question, and an asymmetrical pattern with one path extended to 100 μm for the latter. Axon formation was evaluated by tau-1/MAP2 immunostaining and live-cell imaging of constitutively-active kinesin-1. We found that (1) neurons cannot polarize when extension of all neurites is restricted and that (2) when only a single neurite is permitted to grow long, neurons polarize and the longest neurite becomes the axon. These results provide clear evidence that axon outgrowth is required for its specification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.