ArticlePloS one2012
DNA double strand breaks but not interstrand crosslinks prevent progress through meiosis in fully grown mouse oocytes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Distinct characteristics of the DNA damage response in mammalian oocytes.Experimental & molecular medicine · 2024Review
- Xrcc5/Ku80 is required for the repair of DNA damage in fully grown meiotically arrested mammalian oocytes.Cell death & disease · 2023Article
- Article
- DNA damage in preimplantation embryos and gametes: specification, clinical relevance and repair strategies.Human reproduction update · 2022Review
- Cleavage of Early Mouse Embryo with Damaged DNA.International journal of molecular sciences · 2022Article
- Review
- A retrospective study evaluating the impact of scattering radiation from imaging procedures on oocyte quality during ovarian stimulation for fertility preservation in young breast cancer patients.Breast cancer research and treatment · 2022Article
- Ageing and ovarian stimulation modulate the relative levels of transcript abundance of oocyte DNA repair genes during the germinal vesicle-metaphase II transition in mice.Journal of assisted reproduction and genetics · 2021Article
- Pathogenic Variations of Homologous Recombination GeneFrontiers in cell and developmental biology · 2021Article
- Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes.International journal of molecular sciences · 2020Article
- Regulation of the meiotic divisions of mammalian oocytes and eggs.Biochemical Society transactions · 2018Review
- The capacity of oocytes for DNA repair.Cellular and molecular life sciences : CMLS · 2018Review
- Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes.Scientific reports · 2018Article
- DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes.PloS one · 2017Article
- Maternal Setdb1 Is Required for Meiotic Progression and Preimplantation Development in Mouse.PLoS genetics · 2016Article
- DNA damage response during mouse oocyte maturation.Cell cycle (Georgetown, Tex.) · 2016Article
- DNA damage induces a meiotic arrest in mouse oocytes mediated by the spindle assembly checkpoint.Nature communications · 2015Article
- DNA damage-induced metaphase I arrest is mediated by the spindle assembly checkpoint and maternal age.Nature communications · 2015Article
- Meiotic interstrand DNA damage escapes paternal repair and causes chromosomal aberrations in the zygote by maternal misrepair.Scientific reports · 2015Article
- Cumulus Cells Block Oocyte Meiotic Resumption via Gap Junctions in Cumulus Oocyte Complexes Subjected to DNA Double-Strand Breaks.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is some interest in how mammalian oocytes respond to different types of DNA damage because of the increasing expectation of fertility preservation in women undergoing chemotherapy. Double strand breaks (DSBs) induced by ionizing radiation and agents such as neocarzinostatin (NCS), and interstrand crosslinks (ICLs) induced by alkylating agents such as mitomycin C (MMC), are toxic DNA lesions that need to be repaired for cell survival. Here we examined the effects of NCS and MMC treatment on oocytes collected from antral follicles in mice, because potentially such oocytes are readily collected from ovaries and do not need to be in vitro grown to achieve meiotic competency. We found that oocytes were sensitive to NCS, such that this ionizing radiation mimetic blocked meiosis I and caused fragmented DNA. In contrast, MMC had no impact on the completion of either meiosis I or II, even at extremely high doses. However, oocytes treated with MMC did show γ-H2AX foci and following their in vitro maturation and parthenogenetic activation the development of the subsequent embryos was severely compromised. Addition of MMC to 1-cell embryos caused a similarly poor level of development, demonstrating oocytes have eventual sensitivity to this ICL-inducing agent but this does not occur during their meiotic division. In oocytes, the association of Fanconi Anemia protein, FANCD2, with sites of ICL lesions was not apparent until entry into the embryonic cell cycle. In conclusion, meiotic maturation of oocytes is sensitive to DSBs but not ICLs. The ability of oocytes to tolerate severe ICL damage and yet complete meiosis, means that this type of DNA lesion goes unrepaired in oocytes but impacts on subsequent embryo quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.