ArticlePloS one2012
Transcriptome analysis of the silkworm (Bombyx mori) by high-throughput RNA sequencing.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Transcriptomic Profiling Provides Insight into the Molecular Basis of Heterosis in Philippine-RearedInsects · 2025Article
- Omics in mini-livestock: a genomic perspective on the future of sustainable food systems.Frontiers in genetics · 2025Review
- FOXO-regulated OSER1 reduces oxidative stress and extends lifespan in multiple species.Nature communications · 2024Article
- Article
- Deciphering the Genetic Basis of Silkworm Cocoon Colors Provides New Insights into Biological Coloration and Phenotypic Diversification.Molecular biology and evolution · 2023Article
- Global Profiling of Genes Expressed in the Silk Glands of Philippine-Reared Mulberry SilkwormsInsects · 2022Article
- Comparative Transcriptome Analysis Revealed Genes Involved in Sexual and Polyploid Growth Dimorphisms in Loach (Biology · 2021Article
- An Expanded Survey of the Moth PBP/GOBP Clade inFrontiers in physiology · 2021Article
- Article
- The Bellerophon pipeline, improving de novo transcriptomes and removing chimeras.Ecology and evolution · 2019Article
- Ultrabithorax and abdominal-A specify the abdominal appendage in a dosage-dependent manner in silkworm, Bombyx mori.Heredity · 2017Article
- Article
- Comparative analysis of the integument transcriptomes of the black dilute mutant and the wild-type silkworm Bombyx mori.Scientific reports · 2016Article
- Reassessing ecdysteroidogenic cells from the cell membrane receptors' perspective.Scientific reports · 2016Article
- A comprehensive view of the web-resources related to sericulture.Database : the journal of biological databases and curation · 2016Review
- Transcriptomic Analysis of Resistant and Susceptible Bombyx mori Strains Following BmNPV Infection Provides Insights into the Antiviral Mechanisms.International journal of genomics · 2016Article
- Genome-wide identification and characterization of Fox genes in the silkworm, Bombyx mori.Functional & integrative genomics · 2015Review
- Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.).Journal of proteome research · 2015Article
- Comparative transcriptome analyses on silk glands of six silkmoths imply the genetic basis of silk structure and coloration.BMC genomics · 2015Article
- Comparative analysis of the silk gland transcriptomes between the domestic and wild silkworms.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The domestic silkworm, Bombyx mori, is a model insect with important economic value for silk production that also acts as a bioreactor for biomaterial production. The functional complexity of the silkworm transcriptome has not yet been fully elucidated, although genomic sequencing and other tools have been widely used in its study. We explored the transcriptome of silkworm at different developmental stages using high-throughput paired-end RNA sequencing. A total of about 3.3 gigabases (Gb) of sequence was obtained, representing about a 7-fold coverage of the B. mori genome. From the reads that were mapped to the genome sequence; 23,461 transcripts were obtained, 5,428 of them were novel. Of the 14,623 predicted protein-coding genes in the silkworm genome database, 11,884 of them were found to be expressed in the silkworm transcriptome, giving a coverage of 81.3%. A total of 13,195 new exons were detected, of which, 5,911 were found in the annotated genes in the Silkworm Genome Database (SilkDB). An analysis of alternative splicing in the transcriptome revealed that 3,247 genes had undergone alternative splicing. To help with the data analysis, a transcriptome database that integrates our transcriptome data with the silkworm genome data was constructed and is publicly available at http://124.17.27.136/gbrowse2/. To our knowledge, this is the first study to elucidate the silkworm transcriptome using high-throughput RNA sequencing technology. Our data indicate that the transcriptome of silkworm is much more complex than previously anticipated. This work provides tools and resources for the identification of new functional elements and paves the way for future functional genomics studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.