ArticlePloS one2012
Rhamnose-inducible gene expression in Listeria monocytogenes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Robust Inducible Gene Expression in IntracellularACS synthetic biology · 2025Article
- Characterisation of the growth behaviour of Listeria monocytogenes in Listeria synthetic media.Environmental microbiology reports · 2023Article
- Functional Genomics Identified Novel Genes Involved in Growth at Low Temperatures in Listeria monocytogenes.Microbiology spectrum · 2022Article
- Deciphering the global roles of Cold shock proteins inFrontiers in microbiology · 2022Article
- Anaerobic Growth ofmSphere · 2021Article
- Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes.Cell host & microbe · 2020Article
- Home Alone: Elimination of All but One Alternative Sigma Factor inFrontiers in microbiology · 2017Article
- Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles.Nature communications · 2016Article
- Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris.Scientific reports · 2016Article
- An advanced bioinformatics approach for analyzing RNA-seq data reveals sigma H-dependent regulation of competence genes in Listeria monocytogenes.BMC genomics · 2016Article
- PrfA-like transcription factor gene lmo0753 contributes to L-rhamnose utilization in Listeria monocytogenes strains associated with human food-borne infections.Applied and environmental microbiology · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in L. monocytogenes. Transcription initiation from P(rha) is dose dependent, and a concentration as low as 100 µM rhamnose was found sufficient for induction. Moreover, P(rha) is subject to glucose catabolite repression, which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that P(rha) is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible interference by catabolite repression. The P(rha) promoter provides a novel and useful tool for triggering gene expression in extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.