ArticlePloS one2012
A genetic investigation of the KEOPS complex in halophilic Archaea.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- Article
- A paralog of Pcc1 is the fifth core subunit of the KEOPS tRNA-modifying complex in Archaea.Nature communications · 2023Article
- The universal Sua5/TsaC family evolved different mechanisms for the synthesis of a key tRNA modification.Frontiers in microbiology · 2023Article
- Conservation and Diversification of tRNA tInternational journal of molecular sciences · 2022Review
- The structural and functional workings of KEOPS.Nucleic acids research · 2021Review
- A substrate binding model for the KEOPS tRNA modifying complex.Nature communications · 2020Article
- Monitoring the 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification in eukaryotic tRNAs via the γ-toxin endonuclease.RNA (New York, N.Y.) · 2018Article
- Protein phosphorylation and its role in archaeal signal transduction.FEMS microbiology reviews · 2016Review
- Global translational impacts of the loss of the tRNA modification tMicrobial cell (Graz, Austria) · 2016Article
- Essentiality of threonylcarbamoyladenosine (t(6)A), a universal tRNA modification, in bacteria.Molecular microbiology · 2015Article
- The Levels of a Universally Conserved tRNA Modification Regulate Cell Growth.The Journal of biological chemistry · 2015Article
- Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes.RNA (New York, N.Y.) · 2015Article
- Discovery of the β-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs.Nucleic acids research · 2014Article
- Diversity of the biosynthesis pathway for threonylcarbamoyladenosine (t(6)A), a universal modification of tRNA.RNA biology · 2014Review
- Functional assignment of KEOPS/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification.Nucleic acids research · 2013Article
- A halocin-H4 mutant Haloferax mediterranei strain retains the ability to inhibit growth of other halophilic archaea.Extremophiles : life under extreme conditions · 2013Article
- Post-translation modification in Archaea: lessons from Haloferax volcanii and other haloarchaea.FEMS microbiology reviews · 2013Review
- In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya.Nucleic acids research · 2013Article
- Deinococcus radiodurans YgjD and YeaZ are involved in the repair of DNA cross-links.Extremophiles : life under extreme conditions · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
KEOPS is an important cellular complex conserved in Eukarya, with some subunits conserved in Archaea and Bacteria. This complex was recently found to play an essential role in formation of the tRNA modification threonylcarbamoyladenosine (t(6)A), and was previously associated with telomere length maintenance and transcription. KEOPS subunits are conserved in Archaea, especially in the Euryarchaea, where they had been studied in vitro. Here we attempted to delete the genes encoding the four conserved subunits of the KEOPS complex in the euryarchaeote Haloferax volcanii and study their phenotypes in vivo. The fused kae1-bud32 gene was shown to be essential as was cgi121, which is dispensable in yeast. In contrast, pcc1 (encoding the putative dimerizing unit of KEOPS) was not essential in H. volcanii. Deletion of pcc1 led to pleiotropic phenotypes, including decreased growth rate, reduced levels of t(6)A modification, and elevated levels of intra-cellular glycation products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.