ArticlePloS one2012
Shelf-life evaluation of bilayered human skin equivalent, MyDerm™.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells.Frontiers in medicine · 2021Pooled it
- Improving Wound Healing with DICER1-Modified Keratinocytes.ACS omega · 2025Article
- Enhancing Wound Healing Through Secretome-Loaded 3D-Printed Biomaterials.Gels (Basel, Switzerland) · 2025Review
- Blood Plasma, Fibrinogen or Fibrin Biomaterial for the Manufacturing of Skin Tissue-Engineered Products and Other Dermatological Treatments: A Systematic Review.Journal of functional biomaterials · 2025Review
- Effect of Multiple-Cycle Collections of Conditioned Media from Different Cell Sources towards Fibroblasts in In Vitro Wound Healing Model.Pharmaceutics · 2024Article
- Biological properties and characterization of several variations of a clinical human plasma-based skin substitute model and its manufacturing process.Regenerative biomaterials · 2024Article
- Quality management overview for the production of a tissue-engineered human skin substitute in Malaysia.Stem cell research & therapy · 2023Review
- Plasma-Polymerised Antibacterial Coating of Ovine Tendon Collagen Type I (OTC) Crosslinked with Genipin (GNP) and Dehydrothermal-Crosslinked (DHT) as a Cutaneous Substitute for Wound Healing.Materials (Basel, Switzerland) · 2023Article
- The Effect of Hydroxytyrosol in Type II Epithelial-Mesenchymal Transition in Human Skin Wound Healing.Molecules (Basel, Switzerland) · 2023Article
- Human epidermal keratinocytes and human dermal fibroblasts interactions seeded on gelatin hydrogel for future application in skinFrontiers in bioengineering and biotechnology · 2023Article
- Donor Age and Time in Culture Affect Dermal Fibroblast Contraction in anTissue engineering. Part A · 2022Article
- Article
- Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review.International journal of molecular sciences · 2022Review
- Design and characterization of 3D printed, neomycin-eluting poly-L-lactide mats for wound-healing applications.Journal of materials science. Materials in medicine · 2021Article
- Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application.Polymers · 2021Article
- Advances in Skin Tissue Bioengineering and the Challenges of Clinical Translation.Frontiers in surgery · 2021Review
- Review
- Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing.International journal of molecular sciences · 2020Article
- Review
- In vivo evaluation of bacterial cellulose/acrylic acid wound dressing hydrogel containing keratinocytes and fibroblasts for burn wounds.Drug delivery and translational research · 2019Article
Corrections and comments
- Erratum issuedMaarof, Manira [corrected to Manira, Maarof]
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skin plays an important role in defense against infection and other harmful biological agents. Due to its fragile structure, skin can be easily damaged by heat, chemicals, traumatic injuries and diseases. An autologous bilayered human skin equivalent, MyDerm™, was engineered to provide a living skin substitute to treat critical skin loss. However, one of the disadvantages of living skin substitute is its short shelf-life, hence limiting its distribution worldwide. The aim of this study was to evaluate the shelf-life of MyDerm™ through assessment of cell morphology, cell viability, population doubling time and functional gene expression levels before transplantation. Skin samples were digested with 0.6% Collagenase Type I followed by epithelial cells dissociation with TrypLE Select. Dermal fibroblasts and keratinocytes were culture-expanded to obtain sufficient cells for MyDerm™ construction. MyDerm™ was constructed with plasma-fibrin as temporary biomaterial and evaluated at 0, 24, 48 and 72 hours after storage at 4°C for its shelf-life determination. The morphology of skin cells derived from MyDerm™ remained unchanged across storage times. Cells harvested from MyDerm™ after storage appeared in good viability (90.5%±2.7% to 94.9%±1.6%) and had short population doubling time (58.4±8.7 to 76.9±19 hours). The modest drop in cell viability and increased in population doubling time at longer storage duration did not demonstrate a significant difference. Gene expression for CK10, CK14 and COL III were also comparable between different storage times. In conclusion, MyDerm™ can be stored in basal medium at 4°C for at least 72 hours before transplantation without compromising its functionality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.