ArticlePLoS pathogens2012
A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Pseudomonas aeruginosa Impairs Mitochondrial Function During Infection of Corneal Epithelial Cells.Investigative ophthalmology & visual science · 2026Article
- Altered distribution of chronicity-deficientMicrobiology (Reading, England) · 2026Article
- Interkingdom signaling elicited by bacterial extracellular vesicles in human cystic fibrosis airway epithelium and neutrophils.Frontiers in cellular and infection microbiology · 2026Article
- Structural and functional insights into Pseudomonas aeruginosa's secretion systems 1-6: regulation, role in microbial keratitis and drug targets.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Two Different Isocitrate Dehydrogenases fromInternational journal of molecular sciences · 2023Article
- An anti-biofilm cyclic peptide targets a secreted aminopeptidase from P. aeruginosa.Nature chemical biology · 2023Article
- Meta-omics approaches reveal unique small RNAs exhibited by the uncultured microorganisms dwelling deep-sea hydrothermal sediment in Guaymas Basin.Archives of microbiology · 2022Article
- Biofilm Maintenance as an Active Process: Evidence that Biofilms Work Hard to Stay Put.Journal of bacteriology · 2022Review
- Phylogenetic Classification and Functional Review of Autotransporters.Frontiers in immunology · 2022Review
- Sub-Inhibitory Antibiotic Exposure and Virulence inAntibiotics (Basel, Switzerland) · 2021Review
- The mRNA derived MalH sRNA contributes to alternative carbon source utilization by tuning maltoporin expression in E. coli.RNA biology · 2021Article
- Gene Expression Profiling ofFrontiers in microbiology · 2021Article
- Pseudomonas aeruginosa core metabolism exerts a widespread growth-independent control on virulence.Scientific reports · 2020Article
- An M29 Aminopeptidase fromMicroorganisms · 2020Article
- Outer membrane vesicles for vaccination and targeted drug delivery.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- Catabolism of Nucleic Acids by a Cystic FibrosisFrontiers in microbiology · 2019Article
- Type V Secretion Systems: An Overview of Passenger Domain Functions.Frontiers in microbiology · 2019Review
- Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN.Nucleic acids research · 2018Article
- Article
- The detrimental impact of extracellular bacterial proteases on wound healing.International wound journal · 2017Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
The opportunistic human pathogen, Pseudomonas aeruginosa, is a major cause of infections in chronic wounds, burns and the lungs of cystic fibrosis patients. The P. aeruginosa genome encodes at least three proteins exhibiting the characteristic three domain structure of autotransporters, but much remains to be understood about the functions of these three proteins and their role in pathogenicity. Autotransporters are the largest family of secreted proteins in Gram-negative bacteria, and those characterised are virulence factors. Here, we demonstrate that the PA0328 autotransporter is a cell-surface tethered, arginine-specific aminopeptidase, and have defined its active site by site directed mutagenesis. Hence, we have assigned PA0328 with the name AaaA, for arginine-specific autotransporter of P. aeruginosa. We show that AaaA provides a fitness advantage in environments where the sole source of nitrogen is peptides with an aminoterminal arginine, and that this could be important for establishing an infection, as the lack of AaaA led to attenuation in a mouse chronic wound infection which correlated with lower levels of the cytokines TNFα, IL-1α, KC and COX-2. Consequently AaaA is an important virulence factor playing a significant role in the successful establishment of P. aeruginosa infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.