ArticleMolecular and cellular biology2012
RBF binding to both canonical E2F targets and noncanonical targets depends on functional dE2F/dDP complexes.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 58 citations in OpenAlex.
- Retinoblastoma protein activity revealed by CRISPRi study of divergent Rbf1 and Rbf2 paralogs.G3 (Bethesda, Md.) · 2024Article
- E2F regulation of theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis.Cell reports · 2022Article
- Cell cycle expression of polarity genes features Rb targeting of Vang.Cells & development · 2022Article
- Integrated multi-omics analysis of RB-loss identifies widespread cellular programming and synthetic weaknesses.Communications biology · 2021Article
- Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer.Nature communications · 2021Article
- Article
- The Krüppel-like factor Cabut has cell cycle regulatory properties similar to E2F1.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Soft repression: Subtle transcriptional regulation with global impact.BioEssays : news and reviews in molecular, cellular and developmental biology · 2021Article
- Evolutionarily Conserved Roles for Apontic in Induction and Subsequent Decline of Cyclin E Expression.iScience · 2020Article
- Chlamydomonas CHT7 Is Required for an Effective Quiescent State by Regulating Nutrient-Responsive Cell Cycle Gene Expression.The Plant cell · 2020Article
- Diversification of Retinoblastoma Protein Function Associated with Cis and Trans Adaptations.Molecular biology and evolution · 2019Article
- Changes in chromatin accessibility ensure robust cell cycle exit in terminally differentiated cells.PLoS biology · 2019Article
- Pleiotropic role of Drosophila phosphoribosyl pyrophosphate synthetase in autophagy and lysosome homeostasis.PLoS genetics · 2019Article
- Rbf Activates the Myogenic Transcriptional Program to Promote Skeletal Muscle Differentiation.Cell reports · 2019Article
- Nitrogen-dependent coordination of cell cycle, quiescence and TAG accumulation in Chlamydomonas.Biotechnology for biofuels · 2019Review
- RETRACTED: Genome-wide identification of RETINOBLASTOMA RELATED 1 binding sites in Arabidopsis reveals novel DNA damage regulators.PLoS genetics · 2018Article
- Retinoblastoma Intrinsically Regulates Niche Cell Quiescence, Identity, and Niche Number in the Adult Drosophila Testis.Cell reports · 2018Article
- An alternatively spliced form affecting the Marked Box domain of Drosophila E2F1 is required for proper cell cycle regulation.PLoS genetics · 2018Article
- Identification and Functional Characterization of Anti-metastasis and Anti-angiogenic Activities of Triethylene Glycol Derivatives.Frontiers in oncology · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The retinoblastoma (RB) family of proteins regulate transcription. These proteins lack intrinsic DNA-binding activity but are recruited to specific genomic locations through interactions with sequence-specific DNA-binding factors. The best-known target of RB protein (pRB) is the E2F transcription factor; however, many other chromatin-associated proteins have been described that may allow RB family members to act at additional sites. To gain a perspective on the scale of E2F-dependent and E2F-independent functions, we generated genome-wide binding profiles of RBF1 and dE2F proteins in Drosophila larvae. RBF1 and dE2F2 associate with a large number of binding sites at genes with diverse biological functions. In contrast, dE2F1 was detected at a smaller set of promoters, suggesting that it overrides repression by RBF1/dE2F2 at a specific subset of targets. Approximately 15% of RBF1-bound regions lacked consensus E2F-binding motifs. To test whether RBF1 action at these sites is E2F independent, we examined dDP mutant larvae that lack any functional dE2F/dDP heterodimers. As measured by chromatin immunoprecipitation-microarray analysis (ChIP-chip), ChIP-quantitative PCR (qPCR), and cell fractionation, the stable association of RBF1 with chromatin was eliminated in dDP mutants. This requirement for dDP was seen at classic E2F-regulated promoters and at promoters that lacked canonical E2F-binding sites. These results suggest that E2F/DP complexes are essential for all genomic targeting of RBF1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.